Characterization of INF56, a gene expressed during infection structure development of Uromyces appendiculatus

Xiaoling Xuei, Srirama Bhairi, Richard C. Staples, Olen C. Yoder

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

the bean rust fungus, Uromyces appendiculatus, undergoes thigmotropic differentiation to produce infection structures. Six differentiation-specific genes have been isolated and one, INF24, has been characterized [Bhairi et al., Gene 81 (1989) 237-243]. Here, we report the structure of a second gene, INF56, which was subcloned on a 2.6-kb fragment and sequenced. The location of the 1.0-kb INF56 transcript was determined by S1 nuclease protection and primer extension. A TATA box was found 38 bp upstream and a CAAT box 130 bp upstream from the major transcription start point (tsp). The gene contains two open reading frames: ORF2 is nested within ORF1; they share a 67-bp intron. ORF1 encodes a 14.1-kDa polypeptide which has an amino acid sequence rich in Gly, Pro and Ser. It has sequence similarity to a functional domain (V2) of mammalian cytokeratin type II. ORF2 encodes a 10.1-kDa polypeptide which is rich in Pro. It shares similarity with the cell-surface recognition region of chicken fibronectin. Hybrid selection and in vitro translation of the INF56 mRNA yielded two polypeptides of 15.5 and 23 kDa, as estimated by sodium dodecyl sulfate-polyacrylamide-gel electrophoresis. INF56 is constitutively expressed at a low level, but the abundance of its steady-state transcript is upshifted 4.5 h after spore hydration during the period that infection structures are formed.

Original languageEnglish (US)
Pages (from-to)49-55
Number of pages7
JournalGene
Volume110
Issue number1
DOIs
StatePublished - Jan 2 1992
Externally publishedYes

Fingerprint

Infection
Peptides
Genes
TATA Box
Protein Biosynthesis
Keratins
Spores
Fibronectins
Sodium Dodecyl Sulfate
Introns
Open Reading Frames
Polyacrylamide Gel Electrophoresis
Amino Acid Sequence
Chickens
Fungi
In Vitro Techniques

Keywords

  • Bean rust fungus
  • differentiation-specific gene
  • in vitro translation
  • peptide homology
  • regulation
  • sequencing
  • thigmotropism

ASJC Scopus subject areas

  • Genetics

Cite this

Characterization of INF56, a gene expressed during infection structure development of Uromyces appendiculatus. / Xuei, Xiaoling; Bhairi, Srirama; Staples, Richard C.; Yoder, Olen C.

In: Gene, Vol. 110, No. 1, 02.01.1992, p. 49-55.

Research output: Contribution to journalArticle

Xuei, Xiaoling ; Bhairi, Srirama ; Staples, Richard C. ; Yoder, Olen C. / Characterization of INF56, a gene expressed during infection structure development of Uromyces appendiculatus. In: Gene. 1992 ; Vol. 110, No. 1. pp. 49-55.
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