Characterization of intestinal alkaline phosphatase expression and the tumorigenic potential of γ-irradiated HeLa x fibroblast cell hybrids

Marc Mendonca, Ronald J. Antoniono, Kathryn M. Latham, Eric J. Stanbridge, J. Leslie Redpath

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Fusion of tumorigenic HeLa cells with human skin fibroblasts results in genetically stable hybrids which are nontumorigenic and no longer express the HeLa tumor-associated antigen, intestinal alkaline phosphatase (IAP). Previous analysis of spontaneous segregants of the nontumorigenic hybrid have implicated the loss of one copy of human fibroblast chromosome 11 with reexpression of IAP and tumorigenicity. This observation suggests that a putative HeLa tumor suppressor gene(s) is located on chromosome 11 and that this gene may be a negative regulator of the IAP gene. We have isolated several γ-ray-induced mutants (GIMs) of the nontumorigenic HeLa x skin fibroblast hybrid CGL1 that were specifically selected for reexpression of IAP to further investigate the potential linkage between IAP regulation and the putative tumor suppressor locus. The GIMs have a wide range of cell morphology and level of IAP expression (nearly a factor of 40). The tumorigenicity of the GIMs was examined by s.c. injection into nude mice and all were found to be tumorigenic. The tumor volume-doubling time is in the range of 4 to 8 days for all the cell lines; however, the lag time to reach 500 mm3 tumor volume was significantly longer when the GIM IAP activity was low (

Original languageEnglish (US)
Pages (from-to)4455-4462
Number of pages8
JournalCancer Research
Volume51
Issue number16
StatePublished - Aug 15 1991
Externally publishedYes

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Hybrid Cells
Alkaline Phosphatase
Fibroblasts
Chromosomes, Human, Pair 11
Tumor Burden
Skin
Human Chromosomes
Neoplasm Antigens
Tumor Suppressor Genes
HeLa Cells
Nude Mice
Genes
Cell Line
Injections
Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Characterization of intestinal alkaline phosphatase expression and the tumorigenic potential of γ-irradiated HeLa x fibroblast cell hybrids. / Mendonca, Marc; Antoniono, Ronald J.; Latham, Kathryn M.; Stanbridge, Eric J.; Redpath, J. Leslie.

In: Cancer Research, Vol. 51, No. 16, 15.08.1991, p. 4455-4462.

Research output: Contribution to journalArticle

Mendonca, Marc ; Antoniono, Ronald J. ; Latham, Kathryn M. ; Stanbridge, Eric J. ; Redpath, J. Leslie. / Characterization of intestinal alkaline phosphatase expression and the tumorigenic potential of γ-irradiated HeLa x fibroblast cell hybrids. In: Cancer Research. 1991 ; Vol. 51, No. 16. pp. 4455-4462.
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