Characterization of intraocular immunopathology following intracameral inoculation with alloantigen

Daniel R. Saban, Ian A. Elder, Cuong Q. Nguyen, W. Clay Smith, Adrian M. Timmers, Maria B. Grant, Ammon B. Peck

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Purpose: Anterior chamber-associated immune deviation (ACAID) is a form of peripheral tolerance achieved via intracameral antigen inoculation. It is well known that ACAID effectively down-regulates potentially destructive immunities such as delayed-type hypersensitivity (DTH) at extraorbital sites. However, what has not been specifically addressed is whether local intraocular tissues are negatively affected from intracamerally placed antigen. Thus, the current study was undertaken to detect and characterize potential pathological effects on intraocular tissues following intracamenal inoculation with alloantigen. Methods: ACAID induced in C57/BL/6 hosts via intracameral inoculation with allogeneic (BALB/c) splenocytes was confirmed by the absence of DTH reactivity in the periphery. Injuries to the anterior segment and neuroretina following intracameral inoculation were evaluated pathologically via histological evaluation, molecularly via upregulation of glial fibrillary acidic protein (GFAP), and functionally via assessment of photoreceptor degeneration and electroretinogram (ERG) out to 24 days. In all experiments, intracamerally inoculated mice were compared to sham-operated, and controlled lens-punctured mice - a procedure that elicits intracameral inflammation for positive identification of immunopathological changes. Results: Inflammation of anterior segment tissues persisted out to eight days, despite evidence that significant clearance of allogeneic cells took place within 6 h. In the neuroretina, a transient loss in ERG B-wave amplitudes was detected, but photoreceptor degeneration and GFAP upregulation were not. Conclusions: Intracameral inoculation with alloantigen leads to anterior segment inflammation and ERG dysfunction; however, this was markedly reduced and transient when compared to strong anterior segment inflammation induced by a more serious lens-puncture wound.

Original languageEnglish (US)
Pages (from-to)615-624
Number of pages10
JournalMolecular Vision
Volume14
StatePublished - Mar 26 2008
Externally publishedYes

Fingerprint

Isoantigens
Anterior Chamber
Inflammation
Glial Fibrillary Acidic Protein
Delayed Hypersensitivity
Lenses
Up-Regulation
Peripheral Tolerance
Antigens
Wounds and Injuries
Punctures
Immunity
Down-Regulation

ASJC Scopus subject areas

  • Ophthalmology

Cite this

Saban, D. R., Elder, I. A., Nguyen, C. Q., Smith, W. C., Timmers, A. M., Grant, M. B., & Peck, A. B. (2008). Characterization of intraocular immunopathology following intracameral inoculation with alloantigen. Molecular Vision, 14, 615-624.

Characterization of intraocular immunopathology following intracameral inoculation with alloantigen. / Saban, Daniel R.; Elder, Ian A.; Nguyen, Cuong Q.; Smith, W. Clay; Timmers, Adrian M.; Grant, Maria B.; Peck, Ammon B.

In: Molecular Vision, Vol. 14, 26.03.2008, p. 615-624.

Research output: Contribution to journalArticle

Saban, DR, Elder, IA, Nguyen, CQ, Smith, WC, Timmers, AM, Grant, MB & Peck, AB 2008, 'Characterization of intraocular immunopathology following intracameral inoculation with alloantigen', Molecular Vision, vol. 14, pp. 615-624.
Saban DR, Elder IA, Nguyen CQ, Smith WC, Timmers AM, Grant MB et al. Characterization of intraocular immunopathology following intracameral inoculation with alloantigen. Molecular Vision. 2008 Mar 26;14:615-624.
Saban, Daniel R. ; Elder, Ian A. ; Nguyen, Cuong Q. ; Smith, W. Clay ; Timmers, Adrian M. ; Grant, Maria B. ; Peck, Ammon B. / Characterization of intraocular immunopathology following intracameral inoculation with alloantigen. In: Molecular Vision. 2008 ; Vol. 14. pp. 615-624.
@article{f2e09734377741d392d1098146fb23e1,
title = "Characterization of intraocular immunopathology following intracameral inoculation with alloantigen",
abstract = "Purpose: Anterior chamber-associated immune deviation (ACAID) is a form of peripheral tolerance achieved via intracameral antigen inoculation. It is well known that ACAID effectively down-regulates potentially destructive immunities such as delayed-type hypersensitivity (DTH) at extraorbital sites. However, what has not been specifically addressed is whether local intraocular tissues are negatively affected from intracamerally placed antigen. Thus, the current study was undertaken to detect and characterize potential pathological effects on intraocular tissues following intracamenal inoculation with alloantigen. Methods: ACAID induced in C57/BL/6 hosts via intracameral inoculation with allogeneic (BALB/c) splenocytes was confirmed by the absence of DTH reactivity in the periphery. Injuries to the anterior segment and neuroretina following intracameral inoculation were evaluated pathologically via histological evaluation, molecularly via upregulation of glial fibrillary acidic protein (GFAP), and functionally via assessment of photoreceptor degeneration and electroretinogram (ERG) out to 24 days. In all experiments, intracamerally inoculated mice were compared to sham-operated, and controlled lens-punctured mice - a procedure that elicits intracameral inflammation for positive identification of immunopathological changes. Results: Inflammation of anterior segment tissues persisted out to eight days, despite evidence that significant clearance of allogeneic cells took place within 6 h. In the neuroretina, a transient loss in ERG B-wave amplitudes was detected, but photoreceptor degeneration and GFAP upregulation were not. Conclusions: Intracameral inoculation with alloantigen leads to anterior segment inflammation and ERG dysfunction; however, this was markedly reduced and transient when compared to strong anterior segment inflammation induced by a more serious lens-puncture wound.",
author = "Saban, {Daniel R.} and Elder, {Ian A.} and Nguyen, {Cuong Q.} and Smith, {W. Clay} and Timmers, {Adrian M.} and Grant, {Maria B.} and Peck, {Ammon B.}",
year = "2008",
month = "3",
day = "26",
language = "English (US)",
volume = "14",
pages = "615--624",
journal = "Molecular Vision",
issn = "1090-0535",

}

TY - JOUR

T1 - Characterization of intraocular immunopathology following intracameral inoculation with alloantigen

AU - Saban, Daniel R.

AU - Elder, Ian A.

AU - Nguyen, Cuong Q.

AU - Smith, W. Clay

AU - Timmers, Adrian M.

AU - Grant, Maria B.

AU - Peck, Ammon B.

PY - 2008/3/26

Y1 - 2008/3/26

N2 - Purpose: Anterior chamber-associated immune deviation (ACAID) is a form of peripheral tolerance achieved via intracameral antigen inoculation. It is well known that ACAID effectively down-regulates potentially destructive immunities such as delayed-type hypersensitivity (DTH) at extraorbital sites. However, what has not been specifically addressed is whether local intraocular tissues are negatively affected from intracamerally placed antigen. Thus, the current study was undertaken to detect and characterize potential pathological effects on intraocular tissues following intracamenal inoculation with alloantigen. Methods: ACAID induced in C57/BL/6 hosts via intracameral inoculation with allogeneic (BALB/c) splenocytes was confirmed by the absence of DTH reactivity in the periphery. Injuries to the anterior segment and neuroretina following intracameral inoculation were evaluated pathologically via histological evaluation, molecularly via upregulation of glial fibrillary acidic protein (GFAP), and functionally via assessment of photoreceptor degeneration and electroretinogram (ERG) out to 24 days. In all experiments, intracamerally inoculated mice were compared to sham-operated, and controlled lens-punctured mice - a procedure that elicits intracameral inflammation for positive identification of immunopathological changes. Results: Inflammation of anterior segment tissues persisted out to eight days, despite evidence that significant clearance of allogeneic cells took place within 6 h. In the neuroretina, a transient loss in ERG B-wave amplitudes was detected, but photoreceptor degeneration and GFAP upregulation were not. Conclusions: Intracameral inoculation with alloantigen leads to anterior segment inflammation and ERG dysfunction; however, this was markedly reduced and transient when compared to strong anterior segment inflammation induced by a more serious lens-puncture wound.

AB - Purpose: Anterior chamber-associated immune deviation (ACAID) is a form of peripheral tolerance achieved via intracameral antigen inoculation. It is well known that ACAID effectively down-regulates potentially destructive immunities such as delayed-type hypersensitivity (DTH) at extraorbital sites. However, what has not been specifically addressed is whether local intraocular tissues are negatively affected from intracamerally placed antigen. Thus, the current study was undertaken to detect and characterize potential pathological effects on intraocular tissues following intracamenal inoculation with alloantigen. Methods: ACAID induced in C57/BL/6 hosts via intracameral inoculation with allogeneic (BALB/c) splenocytes was confirmed by the absence of DTH reactivity in the periphery. Injuries to the anterior segment and neuroretina following intracameral inoculation were evaluated pathologically via histological evaluation, molecularly via upregulation of glial fibrillary acidic protein (GFAP), and functionally via assessment of photoreceptor degeneration and electroretinogram (ERG) out to 24 days. In all experiments, intracamerally inoculated mice were compared to sham-operated, and controlled lens-punctured mice - a procedure that elicits intracameral inflammation for positive identification of immunopathological changes. Results: Inflammation of anterior segment tissues persisted out to eight days, despite evidence that significant clearance of allogeneic cells took place within 6 h. In the neuroretina, a transient loss in ERG B-wave amplitudes was detected, but photoreceptor degeneration and GFAP upregulation were not. Conclusions: Intracameral inoculation with alloantigen leads to anterior segment inflammation and ERG dysfunction; however, this was markedly reduced and transient when compared to strong anterior segment inflammation induced by a more serious lens-puncture wound.

UR - http://www.scopus.com/inward/record.url?scp=42349086175&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=42349086175&partnerID=8YFLogxK

M3 - Article

C2 - 18385797

AN - SCOPUS:42349086175

VL - 14

SP - 615

EP - 624

JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

ER -