Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy

Wenqing Shi, Yuhan Zeng, Cheng Zhu, Yucheng Xiao, Theodore Cummins, Jianghui Hou, Lane A. Baker

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Integration of dual-barrel membrane patch-ion channel probes (MP-ICPs) to scanning ion conductance microscopy (SICM) holds promise of providing a revolutionized approach of spatially resolved chemical sensing. A series of experiments are performed to further the understanding of the system and to answer some fundamental questions, in preparation for future developments of this approach. First, MP-ICPs are constructed that contain different types of ion channels including transient receptor potential vanilloid 1 and large conductance Ca2+-activated K+ channels to establish the generalizability of the methods. Next, the capability of the MP-ICP platforms in single ion channel activity measurements is proved. In addition, the interplay between the SICM barrel and the ICP barrel is studied. For ion channels gated by uncharged ligands, channel activity at the ICP barrel is unaffected by the SICM barrel potential; whereas for ion channels that are gated by charged ligands, enhanced channel activity can be obtained by biasing the SICM barrel at potentials with opposite polarity to the charge of the ligand molecules. Finally, a proof-of-principle experiment is performed and site-specific molecular/ionic flux sensing is demonstrated at single-ion-channel level, which show that the MP-ICP platform can be used to quantify local molecular/ionic concentrations.

Original languageEnglish (US)
Article number1702945
JournalSmall
Volume14
Issue number18
DOIs
StatePublished - May 3 2018

Fingerprint

Ion Channels
Microscopy
Microscopic examination
Ions
Membranes
Scanning
Ligand-Gated Ion Channels
Ligands
Calcium-Activated Potassium Channels
Experiments
Fluxes
Molecules

Keywords

  • biosensors
  • ion channels
  • scanned probes

ASJC Scopus subject areas

  • Biotechnology
  • Biomaterials
  • Engineering (miscellaneous)

Cite this

Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy. / Shi, Wenqing; Zeng, Yuhan; Zhu, Cheng; Xiao, Yucheng; Cummins, Theodore; Hou, Jianghui; Baker, Lane A.

In: Small, Vol. 14, No. 18, 1702945, 03.05.2018.

Research output: Contribution to journalArticle

Shi, Wenqing ; Zeng, Yuhan ; Zhu, Cheng ; Xiao, Yucheng ; Cummins, Theodore ; Hou, Jianghui ; Baker, Lane A. / Characterization of Membrane Patch-Ion Channel Probes for Scanning Ion Conductance Microscopy. In: Small. 2018 ; Vol. 14, No. 18.
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