Characterization of multiple 12p rearrangements in testicular germ cell tumor cell line 833K and its subclone 64CP by chromosome microdissection

Ruthann I. Blough, Gail H. Vance, Octavian Henegariu, Teresa A. Smolarek, George W. Sledge, Nyla A. Heerema

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Rearrangements of chromosome arm 12p are known to be common in germ cell tumors (GCT). Previous studies, using fluorescence in situ hybridization (FISH) with a whole chromosome 12 painting probe, showed unusual distributions of chromosome 12-derived chromatin in GCT cell line 833K and its cisplatin-resistant subclone, 64CP, located next to AgNOR (silver staining nucleolus organizer regions), some of which were ectopic. In this study, the ectopic stalk regions were shown by FISH to be composed of 18s and 28s rDNA, but were flanked by β-satellite DNA, which may form a barrier around the rDNA. In order to determine the specific origins of the rearranged chromosome 12 segments, three different derived chromosome 12 regions were isolated from 64CP, using chromosomal microdissection. The microdissected fragments were labeled and hybridized by FISH to normal human chromosomes. All three segments localized to distal 12p; 12p12→12pter, but with apparently different breakpoints for each segment. Furthermore, three-color FISH experiments with 12p band-specific probes demonstrated that the derivative chromosome 12 regions in 833K also originate from distal 12p (12p12→p13). These sequences now can be evaluated for degree of overlap or common breakpoints which may be of significance in the development or progression of GCT.

Original languageEnglish (US)
Pages (from-to)24-29
Number of pages6
JournalCancer Genetics and Cytogenetics
Volume106
Issue number1
DOIs
StatePublished - Oct 1 1998

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Chromosomes, Human, Pair 12
Microdissection
Tumor Cell Line
Fluorescence In Situ Hybridization
Chromosomes
Germ Cell and Embryonal Neoplasms
Ribosomal DNA
Chromosome Painting
Satellite DNA
Nucleolus Organizer Region
Silver Staining
Human Chromosomes
Cisplatin
Chromatin
Color
Testicular Germ Cell Tumor

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Characterization of multiple 12p rearrangements in testicular germ cell tumor cell line 833K and its subclone 64CP by chromosome microdissection. / Blough, Ruthann I.; Vance, Gail H.; Henegariu, Octavian; Smolarek, Teresa A.; Sledge, George W.; Heerema, Nyla A.

In: Cancer Genetics and Cytogenetics, Vol. 106, No. 1, 01.10.1998, p. 24-29.

Research output: Contribution to journalArticle

Blough, Ruthann I. ; Vance, Gail H. ; Henegariu, Octavian ; Smolarek, Teresa A. ; Sledge, George W. ; Heerema, Nyla A. / Characterization of multiple 12p rearrangements in testicular germ cell tumor cell line 833K and its subclone 64CP by chromosome microdissection. In: Cancer Genetics and Cytogenetics. 1998 ; Vol. 106, No. 1. pp. 24-29.
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abstract = "Rearrangements of chromosome arm 12p are known to be common in germ cell tumors (GCT). Previous studies, using fluorescence in situ hybridization (FISH) with a whole chromosome 12 painting probe, showed unusual distributions of chromosome 12-derived chromatin in GCT cell line 833K and its cisplatin-resistant subclone, 64CP, located next to AgNOR (silver staining nucleolus organizer regions), some of which were ectopic. In this study, the ectopic stalk regions were shown by FISH to be composed of 18s and 28s rDNA, but were flanked by β-satellite DNA, which may form a barrier around the rDNA. In order to determine the specific origins of the rearranged chromosome 12 segments, three different derived chromosome 12 regions were isolated from 64CP, using chromosomal microdissection. The microdissected fragments were labeled and hybridized by FISH to normal human chromosomes. All three segments localized to distal 12p; 12p12→12pter, but with apparently different breakpoints for each segment. Furthermore, three-color FISH experiments with 12p band-specific probes demonstrated that the derivative chromosome 12 regions in 833K also originate from distal 12p (12p12→p13). These sequences now can be evaluated for degree of overlap or common breakpoints which may be of significance in the development or progression of GCT.",
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