Characterization of normal human CD3+CD5- and γδ T cell receptor positive T lymphocytes

Edward Srour, T. Leemhuis, L. Jenski, R. Redmond, D. Fillak, J. Jansen

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The functional and phenotypic properties of normal human CD3+CD5- T cells which have a higher frequency of cytotoxic cells than CD3+CD5+ T lymphocytes have been described. Using three- and four-colour immunofluorescence flow cytometric cell sorting, the CD3+CD5- and CD3+CD5+ populations were subdivided into αβ or γδ T cell receptor positive cells. The four subsets were examined for the in vitro cytotoxic activity and were also stimulated with mitogens in limiting-dilution assays to measure the frequencies of proliferating and interleukin-2 (IL-2) producing cells. CD3+CD5-αβ+, CD3+CD5-γδ+ and CD3+CD5+γδ+ cells had lower frequencies of proliferating and IL-2-producing cells than did D3+CD5+αβ+ cells. However, the cytotoxic activity of the different phenotypes was higher in the CD3+CD5- subsets, especially when these cells were γδ+. Expression of γδ or lack of expression of CD5 appeared to be associated with the acquisition of cytolytic potentials. CD8 was expressed on 20% of fresh CD3+γδ+ cells. Cultured γδ+ cells retained the expression of γδ, but quickly lost that of CD8 and with time modulated the expression of CD5. The expression of CD5 was found to be higher on sorted CD3+CD5+γδ- than on CD3+CD5+γδ+ cells. These observations indicate that γδ is preferentially expressed on CD5-negative or weakly positive T lymphocytes and that CD3+CD5-γδ+ cells appear to constitute a discrete small subset of mature T lymphocytes which are cytotoxic in nature. However, the exact immunological function of these cells and their place in T cell ontogeny are yet to be elucidated.

Original languageEnglish
Pages (from-to)114-121
Number of pages8
JournalClinical and Experimental Immunology
Volume80
Issue number1
StatePublished - 1990

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T-Cell Antigen Receptor
T-Lymphocytes
Interleukin-2
Color
T-Lymphocyte Subsets
Cytotoxic T-Lymphocytes
Mitogens
Fluorescent Antibody Technique
Cultured Cells
Phenotype

Keywords

  • γδ T cell receptor
  • CD3CD5

ASJC Scopus subject areas

  • Immunology

Cite this

Srour, E., Leemhuis, T., Jenski, L., Redmond, R., Fillak, D., & Jansen, J. (1990). Characterization of normal human CD3+CD5- and γδ T cell receptor positive T lymphocytes. Clinical and Experimental Immunology, 80(1), 114-121.

Characterization of normal human CD3+CD5- and γδ T cell receptor positive T lymphocytes. / Srour, Edward; Leemhuis, T.; Jenski, L.; Redmond, R.; Fillak, D.; Jansen, J.

In: Clinical and Experimental Immunology, Vol. 80, No. 1, 1990, p. 114-121.

Research output: Contribution to journalArticle

Srour, E, Leemhuis, T, Jenski, L, Redmond, R, Fillak, D & Jansen, J 1990, 'Characterization of normal human CD3+CD5- and γδ T cell receptor positive T lymphocytes', Clinical and Experimental Immunology, vol. 80, no. 1, pp. 114-121.
Srour, Edward ; Leemhuis, T. ; Jenski, L. ; Redmond, R. ; Fillak, D. ; Jansen, J. / Characterization of normal human CD3+CD5- and γδ T cell receptor positive T lymphocytes. In: Clinical and Experimental Immunology. 1990 ; Vol. 80, No. 1. pp. 114-121.
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