Characterization of skeletal muscle effects associated with daptomycin in rats

Tatiana Y. Kostrominova, Cheryl A. Hassett, Erik P. Rader, Carol Davis, Lisa M. Larkin, Scott Coleman, Frederick B. Oleson, John A. Faulkner

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Daptomycin is a lipopeptide antibiotic with strong bactericidal effects against Gram-positive bacteria and minor side effects on skeletal muscles. The type and magnitude of the early effect of daptomycin on skeletal muscles of rats was quantified by histopathology, examination of contractile properties, Evans Blue Dye uptake, and effect on the patch repair process. A single dose of daptomycin of up to 200 mg/kg had no effect on muscle fibers. A dose of 150 mg/kg of daptomycin, twice per day for 3 days, produced a small number of myofibers (≤0.22%) with loss of plasma membrane integrity and/or infiltration by neutrophils and/or macrophages. Multiple doses of daptomycin are required for a quantifiable effect on skeletal muscles of rats. Some fibers were Evans Blue Dye-positive but were not yet infiltrated by neutrophils. This suggests that the sarcolemma may be the primary target for the observed effects.

Original languageEnglish (US)
Pages (from-to)385-393
Number of pages9
JournalMuscle and Nerve
Volume42
Issue number3
DOIs
StatePublished - Sep 2010

Keywords

  • Caveolin-3
  • Daptomycin
  • Dysferlin
  • LAMP-1
  • Sarcolemma

ASJC Scopus subject areas

  • Physiology
  • Clinical Neurology
  • Cellular and Molecular Neuroscience
  • Physiology (medical)

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