Characterization of the 5′-end region and the first two exons of the β-protein precursor gene

Giuseppe La Fauci, Debomoy Lahiri, Stephen R J Salton, Nikolaos K. Robakis

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Human genomic clones encoding the promoter region and the first two exons of the β-amyloid protein precursor (β-APP) gene were isolated. The first exon is 205 base pairs (bp) long and encodes 19 amino acids. The second exon is 168 bp long and encodes 56 amino acids. The 5′-flanking sequence of the β-APP gene was found to display promoter activity in several cell lines including PC12 cells where the highest activity was detected. The promoter region of this gene lacks the typical "TATAA" and "CAAT" boxes usually associated with eukaryotic promoters. Five copies of the GGGCGC sequence are located between positions -107 and -188 and one copy is located within the first exon of the β-APP gene. Consensus sequences recognized by the transcription factors Sp1 and AP-1 are located upstream from the RNA start site. Palindromic sequences capable of forming stable hairpin-like structures are found aroud the main transcription initiation site. The structural characteristics of the β-APP promoter indicate that multiple elements participate in the regulation of the expression of this gene.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number1
DOIs
StatePublished - Feb 28 1989
Externally publishedYes

Fingerprint

Protein Precursors
Exons
Genes
Genetic Promoter Regions
Base Pairing
Amino Acids
Amyloid beta-Protein Precursor
5' Flanking Region
Transcription Initiation Site
PC12 Cells
Sp1 Transcription Factor
Transcription Factor AP-1
Consensus Sequence
Gene Expression Regulation
Clone Cells
RNA
Cell Line
Cells

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Characterization of the 5′-end region and the first two exons of the β-protein precursor gene. / La Fauci, Giuseppe; Lahiri, Debomoy; Salton, Stephen R J; Robakis, Nikolaos K.

In: Biochemical and Biophysical Research Communications, Vol. 159, No. 1, 28.02.1989, p. 297-304.

Research output: Contribution to journalArticle

La Fauci, Giuseppe ; Lahiri, Debomoy ; Salton, Stephen R J ; Robakis, Nikolaos K. / Characterization of the 5′-end region and the first two exons of the β-protein precursor gene. In: Biochemical and Biophysical Research Communications. 1989 ; Vol. 159, No. 1. pp. 297-304.
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