Characterization of the 5′-end region and the first two exons of the β-protein precursor gene

Giuseppe La Fauci, Debomoy K. Lahiri, Stephen R.J. Salton, Nikolaos K. Robakis

Research output: Contribution to journalArticle

57 Scopus citations

Abstract

Human genomic clones encoding the promoter region and the first two exons of the β-amyloid protein precursor (β-APP) gene were isolated. The first exon is 205 base pairs (bp) long and encodes 19 amino acids. The second exon is 168 bp long and encodes 56 amino acids. The 5′-flanking sequence of the β-APP gene was found to display promoter activity in several cell lines including PC12 cells where the highest activity was detected. The promoter region of this gene lacks the typical "TATAA" and "CAAT" boxes usually associated with eukaryotic promoters. Five copies of the GGGCGC sequence are located between positions -107 and -188 and one copy is located within the first exon of the β-APP gene. Consensus sequences recognized by the transcription factors Sp1 and AP-1 are located upstream from the RNA start site. Palindromic sequences capable of forming stable hairpin-like structures are found aroud the main transcription initiation site. The structural characteristics of the β-APP promoter indicate that multiple elements participate in the regulation of the expression of this gene.

Original languageEnglish (US)
Pages (from-to)297-304
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume159
Issue number1
DOIs
StatePublished - Feb 28 1989
Externally publishedYes

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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