Characterization of the 5′-flanking region of the human tartrate-resistant acid phosphatase (TRAP) gene

S. V. Reddy, N. Kuzhandaivelu, L. G. Acosta, G. David Roodman

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Tartrate-resistant acid phosphatase (TRAP) is expressed at high levels in osteoclasts and may play an important role in the bone resorptive process. However, factors regulating human TRAP gene expression have not been clearly defined. Therefore, we isolated a genomic clone (CL-9) for TRAP containing a 14-kb insert. A restriction map was generated for this insert, and a 2.6-kb Apal fragment containing the 5′-flanking region was subcloned. Sequence analysis of this fragment revealed the presence of candidate transcription factor-binding sequences for H-APF-1, SP1, GATA2, and the c-Myc proto-oncogene. PCR analysis of RNA isolated from human osteoclastomas and pagetic bone revealed a 276-bp intron at -1 by to -276 bp relative to the ATG and a transcript originating from this intron. Rapid amplification of the 5′ end of the human TRAP mRNA by PCR indicated the presence of a 93-bp untranslated region 5′ from the intron. Promoter activity was detected in the DNA fragment from +1 bp to -1903 by relative to the ATG initiation codon, which drove the transient expression of a luciferase reporter gene when transfected into HRE H9 rabbit endometrial cells. Comparison of the human TRAP 5′-flanking region with mouse TRAP and uteroferrin revealed 41% and 47% homology, respectively. This suggests that regulation of human TRAP gene expression may differ from that for the murine TRAP gene.

Original languageEnglish (US)
Pages (from-to)587-593
Number of pages7
JournalBone
Volume16
Issue number5
DOIs
StatePublished - 1995
Externally publishedYes

Fingerprint

5' Flanking Region
Genes
Introns
Gene Expression
Tartrate-Resistant Acid Phosphatase
Bone and Bones
Polymerase Chain Reaction
myc Genes
Initiator Codon
5' Untranslated Regions
Osteoclasts
Ubiquitin
Luciferases
Reporter Genes
Sequence Analysis
Transcription Factors
Clone Cells
RNA
Rabbits
Messenger RNA

Keywords

  • 5′-flanking sequence
  • Intron
  • Polymerase chain reaction
  • Promoter
  • Tartrate-resistant acid phosphatase
  • Transcription factors

ASJC Scopus subject areas

  • Physiology
  • Endocrinology, Diabetes and Metabolism
  • Histology
  • Hematology

Cite this

Characterization of the 5′-flanking region of the human tartrate-resistant acid phosphatase (TRAP) gene. / Reddy, S. V.; Kuzhandaivelu, N.; Acosta, L. G.; Roodman, G. David.

In: Bone, Vol. 16, No. 5, 1995, p. 587-593.

Research output: Contribution to journalArticle

Reddy, S. V. ; Kuzhandaivelu, N. ; Acosta, L. G. ; Roodman, G. David. / Characterization of the 5′-flanking region of the human tartrate-resistant acid phosphatase (TRAP) gene. In: Bone. 1995 ; Vol. 16, No. 5. pp. 587-593.
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