Characterization of the 5′-flanking sequence of rat class I alcohol dehydrogenase gene

Joseph F. Cortese, Jessica L. Majewski, David W. Crabb, Howard J. Edenberg, Vincent W. Yang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Expression of the rat class I alcohol dehydrogenase (ADH) gene is highest in the liver and regions of the intestine. We characterized over 3 kilobases of the gene's 5′-flanking region by sequencing and transient transfection. Alignment of the flanking sequence of the rat gene with those of the mouse and human class I genes revealed a cis-acting element, known to be a functional glucocorticoid response element in the human gene and conserved in the mouse, is interrupted in the rat promoter by a 490-base pair processed retropseudogene of the ribosomal protein S25. Southern analysis indicated that this inserted element is present in the class I ADH promoters of multiple strains of rat. Transfection analysis of the rat and mouse promoters showed that the mouse, but not the rat promoter, is inducible by dexamethasone. Electrophoretic mobility shift assays using nuclear extracts from dexamethasone-treated cells confirmed that the mouse's element interacts with the glucocorticoid receptor. Transient transfection of the 5′-flanking region of the rat gene linked to a human growth hormone reporter demonstrated the liver and intestinal specificity of the rat promoter. Two positive elements, one from nucleotides -1,327 to -977 and the other from -241 to -12, were shown to support high levels of reporter activity. In addition, a suppressive element was localized between nucleotides -403 and -241, a region of DNA situated within the domain of the S25 ribosomal protein pseudogene.

Original languageEnglish
Pages (from-to)21898-21906
Number of pages9
JournalJournal of Biological Chemistry
Volume269
Issue number34
StatePublished - Aug 26 1994

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Alcohol Dehydrogenase
5' Flanking Region
Rats
Genes
Transfection
Ribosomal Proteins
Liver
Dexamethasone
Nucleotides
MHC Class I Genes
Electrophoretic mobility
Pseudogenes
Human Growth Hormone
Sequence Alignment
Glucocorticoid Receptors
Response Elements
Electrophoretic Mobility Shift Assay
Base Pairing
Glucocorticoids
Intestines

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of the 5′-flanking sequence of rat class I alcohol dehydrogenase gene. / Cortese, Joseph F.; Majewski, Jessica L.; Crabb, David W.; Edenberg, Howard J.; Yang, Vincent W.

In: Journal of Biological Chemistry, Vol. 269, No. 34, 26.08.1994, p. 21898-21906.

Research output: Contribution to journalArticle

Cortese, Joseph F. ; Majewski, Jessica L. ; Crabb, David W. ; Edenberg, Howard J. ; Yang, Vincent W. / Characterization of the 5′-flanking sequence of rat class I alcohol dehydrogenase gene. In: Journal of Biological Chemistry. 1994 ; Vol. 269, No. 34. pp. 21898-21906.
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