Characterization of the APC gene in sporadic gastric adenocarcinomas

Steven M. Powell, Oscar W. Cummings, Janica A. Mullen, Ali Asghar, Giovanni Fuga, Paolo Piva, Chiara Minacci, Tiziana Megha, Piero Tosi, Charles E. Jackson

Research output: Contribution to journalArticle

25 Scopus citations

Abstract

The prominent role of the APC gene in colorectal tumor development is well established. However, its role in tumorigenesis in other tissues is not clear. Hence, DNA from 30 primary sporadic gastric adenocarcinomas was obtained from patients living in a high risk area of the world (North-Central Italy) in order to further define APC's role in gastric tumorigenesis. We thoroughly examined that region of APC which is commonly mutated in colorectal tumors using proven sensitive methods. The IVS protein assay and DNA sequence analysis of APC codons 686 through 1693 revealed no intragenic mutations. However, allelic loss of loci near APC was detected in 7 (28%) of 25 informative gastric adenocarcinomas using two 5q dinucleotide repeat markers for LOH analysis. These results suggest that genetic alteration of a region of APC commonly mutated in colorectal cancer is not a common event during sporadic gastric tumor development, at least in patients from North-Central Italy. Further analysis of chromosome 5q might identify another gene to be significantly altered in these gastric cancers.

Original languageEnglish (US)
Pages (from-to)1953-1959
Number of pages7
JournalOncogene
Volume12
Issue number9
StatePublished - Jun 3 1996

Keywords

  • Adenomatous polyposis coli (APC)
  • Gastric carcinoma
  • Loss of heterozygosity
  • Molecular genetics
  • Mutations

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research
  • Genetics

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  • Cite this

    Powell, S. M., Cummings, O. W., Mullen, J. A., Asghar, A., Fuga, G., Piva, P., Minacci, C., Megha, T., Tosi, P., & Jackson, C. E. (1996). Characterization of the APC gene in sporadic gastric adenocarcinomas. Oncogene, 12(9), 1953-1959.