Characterization of the C-terminal domain of a potassium channel from Streptomyces lividans (KcsA)

Victor P T Pau, Yongfang Zhu, Zhiguang Yuchi, Quyen Hoang, Daniel S C Yang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

KcsA, a potassium channel from Streptomyces lividans, is a good model for probing the general working mechanism of potassium channels. To date, the physiological activator of KcsA is still unknown, but in vitro studies showed that it could be opened by lowering the pH of the cytoplasmic compartment to 4. The C-terminal domain (CTD, residues 112-160) was proposed to be the modulator for this pH-responsive event. Here, we support this proposal by examining the pH profiles of: (a) thermal stability of KcsA with and without its CTD and (b) aggregation properties of a recombinant fragment of CTD. We found that the presence of the CTD weakened and enhanced the stability of KcsA at acidic and basic pH values, respectively. In addition, the CTD fragment oligomerized at basic pH values with a transition profile close to that of channel opening. Our results are consistent with the CTD being a pH modulator. We propose herein a mechanism on how this domain may contribute to the pH-dependent opening of KcsA.

Original languageEnglish (US)
Pages (from-to)29163-29169
Number of pages7
JournalJournal of Biological Chemistry
Volume282
Issue number40
DOIs
StatePublished - Oct 5 2007
Externally publishedYes

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Streptomyces lividans
Potassium Channels
Modulators
Thermodynamic stability
Agglomeration
Hot Temperature

ASJC Scopus subject areas

  • Biochemistry

Cite this

Characterization of the C-terminal domain of a potassium channel from Streptomyces lividans (KcsA). / Pau, Victor P T; Zhu, Yongfang; Yuchi, Zhiguang; Hoang, Quyen; Yang, Daniel S C.

In: Journal of Biological Chemistry, Vol. 282, No. 40, 05.10.2007, p. 29163-29169.

Research output: Contribution to journalArticle

Pau, Victor P T ; Zhu, Yongfang ; Yuchi, Zhiguang ; Hoang, Quyen ; Yang, Daniel S C. / Characterization of the C-terminal domain of a potassium channel from Streptomyces lividans (KcsA). In: Journal of Biological Chemistry. 2007 ; Vol. 282, No. 40. pp. 29163-29169.
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