Characterization of the cholinergic neuronal differentiation of the human neuroblastoma cell line LA-N-5 after treatment with retinoic acid

David P. Hill, Kent Robertson

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Analysis of the molecular factors that control cellular differentiation in mammalian embryos is difficult due to the small amount of material available from embryos and their inaccessibility during gestation. One way to circumvent these limitations is to use model systems that allow the study of differentiation in vitro. In this study we have characterized the response of a human neuroblastoma cell line, LA-N-5, to the differentiation-inducing agent, all-trans retinoic acid (RA) using 23 markers that are characteristic of neural crest cells and some of their derivatives. Following induction with RA, the neural crest-like LA-N-5 cells undergo differentiation into cholinergic neurons with increased expression of a variety of neural-specific markers including neurofilaments, growth associated protein-43, tetanus toxin binding sites, receptors for neurotrophic factors, neuropeptides, choline acetyl transferase, vesicular acetylcholine transporter, and acetylcholinesterase with a concomitant decrease in the expression of non- neuronal markers. These results provide the basis for the use of retinoic acid-induced differentiation of LA-N-5 cells as a model system to study molecular events associated with the differentiation of cholinergic neurons.

Original languageEnglish
Pages (from-to)53-67
Number of pages15
JournalDevelopmental Brain Research
Volume102
Issue number1
DOIs
StatePublished - Aug 18 1997

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Tretinoin
Neuroblastoma
Cholinergic Agents
Cholinergic Neurons
Neural Crest
Cell Line
Vesicular Acetylcholine Transport Proteins
Mammalian Embryo
GAP-43 Protein
Tetanus Toxin
Nerve Growth Factor Receptors
Intermediate Filaments
Acetylcholinesterase
Transferases
Choline
Neuropeptides
Statistical Factor Analysis
Cell Differentiation
Embryonic Structures
Binding Sites

Keywords

  • Cholinergic neuronal differentiation
  • LA-N- 5
  • Neural crest
  • Neuroblastoma
  • Neuropeptide
  • Neurotrophic factor
  • Retinoic acid

ASJC Scopus subject areas

  • Developmental Biology
  • Developmental Neuroscience

Cite this

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abstract = "Analysis of the molecular factors that control cellular differentiation in mammalian embryos is difficult due to the small amount of material available from embryos and their inaccessibility during gestation. One way to circumvent these limitations is to use model systems that allow the study of differentiation in vitro. In this study we have characterized the response of a human neuroblastoma cell line, LA-N-5, to the differentiation-inducing agent, all-trans retinoic acid (RA) using 23 markers that are characteristic of neural crest cells and some of their derivatives. Following induction with RA, the neural crest-like LA-N-5 cells undergo differentiation into cholinergic neurons with increased expression of a variety of neural-specific markers including neurofilaments, growth associated protein-43, tetanus toxin binding sites, receptors for neurotrophic factors, neuropeptides, choline acetyl transferase, vesicular acetylcholine transporter, and acetylcholinesterase with a concomitant decrease in the expression of non- neuronal markers. These results provide the basis for the use of retinoic acid-induced differentiation of LA-N-5 cells as a model system to study molecular events associated with the differentiation of cholinergic neurons.",
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