Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii

Chao-Hung Lee, Mark E. Lasbury, John R. Paulsrud, Nancy L. Bauer, Stephen L. Brady, Geoffrey A. Weinberg, Pamela J. Durant, Marilyn S. Bartlett, James W. Smitha

Research output: Contribution to journalArticle

Abstract

A cDNA clone derived from Pneumocvstis carinii contained an unusual sequence (GTGATG)2(ATGGTG)4(ATG)4 and many GAT repeats. It was found to encode a histidine and aspartic acid-rich protein (HARP). The complete cDNA contained an 888-bp open reading frame encoding a putative protein of 32.6 kDa. The deduced HARP protein contained 39 aspartic acid and 22 histidine residues. The genomic copy of the HARP gene (1203 bp in length) was found to contain 3 small introns of 46, 44, and 38 bp, respectively. HARP was predicted by computer programs to be a plasma membrane protein with nickel-binding activity.

Original languageEnglish
Pages (from-to)581-584
Number of pages4
JournalJournal of Eukaryotic Microbiology
Volume47
Issue number6
StatePublished - 2000

Fingerprint

Pneumocystis carinii
aspartic acid
histidine
Histidine
Aspartic Acid
Genes
Proteins
genes
proteins
Complementary DNA
Nickel
nickel
membrane proteins
Introns
Open Reading Frames
blood proteins
open reading frames
Pneumocystis carinii histidine-aspartic acid-rich protein
introns
Blood Proteins

Keywords

  • AIDS
  • Glycoprotein A
  • Immunosuppression
  • Major surface glycoprotein
  • Nickel-binding protein
  • Opportunistic pathogen
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Cite this

Lee, C-H., Lasbury, M. E., Paulsrud, J. R., Bauer, N. L., Brady, S. L., Weinberg, G. A., ... Smitha, J. W. (2000). Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii. Journal of Eukaryotic Microbiology, 47(6), 581-584.

Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii. / Lee, Chao-Hung; Lasbury, Mark E.; Paulsrud, John R.; Bauer, Nancy L.; Brady, Stephen L.; Weinberg, Geoffrey A.; Durant, Pamela J.; Bartlett, Marilyn S.; Smitha, James W.

In: Journal of Eukaryotic Microbiology, Vol. 47, No. 6, 2000, p. 581-584.

Research output: Contribution to journalArticle

Lee, C-H, Lasbury, ME, Paulsrud, JR, Bauer, NL, Brady, SL, Weinberg, GA, Durant, PJ, Bartlett, MS & Smitha, JW 2000, 'Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii', Journal of Eukaryotic Microbiology, vol. 47, no. 6, pp. 581-584.
Lee, Chao-Hung ; Lasbury, Mark E. ; Paulsrud, John R. ; Bauer, Nancy L. ; Brady, Stephen L. ; Weinberg, Geoffrey A. ; Durant, Pamela J. ; Bartlett, Marilyn S. ; Smitha, James W. / Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii. In: Journal of Eukaryotic Microbiology. 2000 ; Vol. 47, No. 6. pp. 581-584.
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