Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii

Chao Hung Lee, Mark E. Lasbury, John R. Paulsrud, Nancy L. Bauer, Stephen L. Brady, Geoffrey A. Weinberg, Pamela J. Durant, Marilyn S. Bartlett, James W. Smitha

Research output: Contribution to journalArticle

Abstract

A cDNA clone derived from Pneumocvstis carinii contained an unusual sequence (GTGATG)2(ATGGTG)4(ATG)4 and many GAT repeats. It was found to encode a histidine and aspartic acid-rich protein (HARP). The complete cDNA contained an 888-bp open reading frame encoding a putative protein of 32.6 kDa. The deduced HARP protein contained 39 aspartic acid and 22 histidine residues. The genomic copy of the HARP gene (1203 bp in length) was found to contain 3 small introns of 46, 44, and 38 bp, respectively. HARP was predicted by computer programs to be a plasma membrane protein with nickel-binding activity.

Original languageEnglish (US)
Pages (from-to)581-584
Number of pages4
JournalJournal of Eukaryotic Microbiology
Volume47
Issue number6
DOIs
StatePublished - Jan 1 2000

Keywords

  • AIDS
  • Glycoprotein A
  • Immunosuppression
  • Major surface glycoprotein
  • Nickel-binding protein
  • Opportunistic pathogen
  • Pulsed-field gel electrophoresis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Applied Microbiology and Biotechnology
  • Agricultural and Biological Sciences (miscellaneous)
  • Microbiology

Fingerprint Dive into the research topics of 'Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii'. Together they form a unique fingerprint.

  • Cite this

    Lee, C. H., Lasbury, M. E., Paulsrud, J. R., Bauer, N. L., Brady, S. L., Weinberg, G. A., Durant, P. J., Bartlett, M. S., & Smitha, J. W. (2000). Characterization of the gene encoding a histidine and aspartic acid-rich protein from Pneumocystis carinii. Journal of Eukaryotic Microbiology, 47(6), 581-584. https://doi.org/10.1111/j.1550-7408.2000.tb00093.x