Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers

Chao-Hung Lee, S. L. Moseley, H. W. Moon, S. C. Whipp, C. L. Gyles, M. So

Research output: Contribution to journalArticle

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Abstract

The gene encoding heat-stable toxin II (STII) was cloned into an Escherichia coli K-12 strain, and its nucleotide sequence was determined. The deduced amino acid sequence indicates that SII is synthesized within the cell as a 71-amino-acid protein and that neither the DNA nor amino acid sequence bears any similarity to that of heat-stable toxin I. A DNA fragment containing the SII gene was used to probe enterotoxigenic E. coli clinical isolates with various toxin phenotypes and was shown to be useful in detecting all STII and only STII producers.

Original languageEnglish
Pages (from-to)264-268
Number of pages5
JournalInfection and Immunity
Volume42
Issue number1
StatePublished - 1983

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Enterotoxigenic Escherichia coli
Epidemiologic Studies
Amino Acid Sequence
Hot Temperature
DNA
Genes
Escherichia coli
Phenotype
Amino Acids
Proteins
heat stable toxin (E coli)
scorpion toxin I''

ASJC Scopus subject areas

  • Immunology

Cite this

Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers. / Lee, Chao-Hung; Moseley, S. L.; Moon, H. W.; Whipp, S. C.; Gyles, C. L.; So, M.

In: Infection and Immunity, Vol. 42, No. 1, 1983, p. 264-268.

Research output: Contribution to journalArticle

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