Characterization of the gene encoding heat-stable toxin II and preliminary molecular epidemiological studies of enterotoxigenic Escherichia coli heat-stable toxin II producers

C. H. Lee, S. L. Moseley, H. W. Moon, S. C. Whipp, C. L. Gyles, M. So

Research output: Contribution to journalArticle

80 Scopus citations

Abstract

The gene encoding heat-stable toxin II (STII) was cloned into an Escherichia coli K-12 strain, and its nucleotide sequence was determined. The deduced amino acid sequence indicates that SII is synthesized within the cell as a 71-amino-acid protein and that neither the DNA nor amino acid sequence bears any similarity to that of heat-stable toxin I. A DNA fragment containing the SII gene was used to probe enterotoxigenic E. coli clinical isolates with various toxin phenotypes and was shown to be useful in detecting all STII and only STII producers.

Original languageEnglish (US)
Pages (from-to)264-268
Number of pages5
JournalInfection and immunity
Volume42
Issue number1
StatePublished - Dec 4 1983

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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