Characterization of the highly regulated antigen BBA05 in the enzootic cycle of Borrelia burgdorferi

Haijun Xu, Ming He, Xiujuan Pang, Zao C. Xu, Joseph Piesman, X. Yang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Dramatic alteration of surface lipoprotein profiles is a key strategy that Borrelia burgdorferi, the Lyme disease pathogen, has evolved for adapting to the diverse environments of arthropod and mammalian hosts. Several of these differentially expressed lipoproteins have been shown to play important roles in the enzootic cycle of B. burgdorferi. The BBA05 protein is a previously identified putative lipoprotein (P55 or S1 antigen) that elicits antibody responses in mammals. Recent microarray analyses indicate that the BBA05 gene is differentially expressed by many environmental factors, including temperature. However, the role of the BBA05 protein in the life cycle of B. burgdorferi has not been elucidated. Here we show that expression of the BBA05 gene was exclusively induced in feeding nymphal ticks during the spirochetal transmission from ticks to mammals. Upon generating a BBA05 mutant in an infectious strain of B. burgdorferi, we showed that the BBA05 mutant remained capable of establishing infection in mice, being acquired by ticks, persisting through tick molting, and reinfecting new mammalian hosts. These results indicate that, despite being a highly conserved and regulated antigen, the BBA05 protein has a nonessential role in the transmission cycle of B. burgdorferi, at least in the animal model.

Original languageEnglish
Pages (from-to)100-107
Number of pages8
JournalInfection and Immunity
Volume78
Issue number1
DOIs
StatePublished - Jan 2010

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Borrelia burgdorferi
Ticks
Antigens
Lipoproteins
Mammals
Molting
Proteins
Lyme Disease
Arthropods
Microarray Analysis
Life Cycle Stages
Antibody Formation
Animal Models
Gene Expression
Temperature
Infection
Genes

ASJC Scopus subject areas

  • Immunology
  • Microbiology
  • Parasitology
  • Infectious Diseases

Cite this

Characterization of the highly regulated antigen BBA05 in the enzootic cycle of Borrelia burgdorferi. / Xu, Haijun; He, Ming; Pang, Xiujuan; Xu, Zao C.; Piesman, Joseph; Yang, X.

In: Infection and Immunity, Vol. 78, No. 1, 01.2010, p. 100-107.

Research output: Contribution to journalArticle

Xu, Haijun ; He, Ming ; Pang, Xiujuan ; Xu, Zao C. ; Piesman, Joseph ; Yang, X. / Characterization of the highly regulated antigen BBA05 in the enzootic cycle of Borrelia burgdorferi. In: Infection and Immunity. 2010 ; Vol. 78, No. 1. pp. 100-107.
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