Characterization of the human burst-forming unit-megakaryocyte

R. A. Briddell, J. E. Brandt, J. E. Straneva, Edward Srour, R. Hoffman

Research output: Contribution to journalArticle

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Abstract

Two classes of human marrow megakaryocyte progenitor cells are described. Colony-forming unit-megakaryocyte (CFU-MK)-derived colonies appeared in vitro after 12-day incubation; burst-forming unit-megakaryocyte (BFU-MK)-derived colonies appeared after 21 days. CFU-MK-derived colonies were primarily unifocal and composed of 11.6 ± 1.2 cells/colony; BFU-MK-derived colonies were composed of 2.3 ± 0.4 foci and 108.6 ± 4.4 cells/colony. CFU-MK and BFU-MK were separable by counterflow centrifugal elutriation. CFU-MK colony formation was diminished by exposure to 5-fluorouracil (5-FU); BFU-MK colony formation was unaffected. CFU-MK and BFU-MK were immunologically phenotyped. CFU-MK expressed the human progenitor cell antigen-1 (HPCA-1, CD34, clone My10) and a major histocompatibility class II locus, HLA-DR, and BFU-MK expressed only detectable amounts of CD34. BFU-MK colony formation was entirely dependent on addition of exogeneous hematopoietic growth factors. Recombinant granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-3 (IL-3) possessed such colony-stimulating activity, whereas recombinant erythropoietin (Epo), G-CSF, IL-1α, IL-4, and purified thrombocytopoiesis-stimulating factor did not. These studies indicate the existence of a human megakaryocyte progenitor cell, the BFU-MK, which has unique properties allowing it to be distinguished from the CFU-MK.

Original languageEnglish
Pages (from-to)145-151
Number of pages7
JournalBlood
Volume74
Issue number1
StatePublished - 1989

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Thrombopoietin
Megakaryocytes
Interleukin-3
HLA-DR Antigens
Granulocyte Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor
Erythropoietin
Interleukin-1
Fluorouracil
Interleukin-4
Intercellular Signaling Peptides and Proteins
Antigens
Stem Cells
Megakaryocyte Progenitor Cells
Histocompatibility
Macrophage Colony-Stimulating Factor

ASJC Scopus subject areas

  • Hematology

Cite this

Briddell, R. A., Brandt, J. E., Straneva, J. E., Srour, E., & Hoffman, R. (1989). Characterization of the human burst-forming unit-megakaryocyte. Blood, 74(1), 145-151.

Characterization of the human burst-forming unit-megakaryocyte. / Briddell, R. A.; Brandt, J. E.; Straneva, J. E.; Srour, Edward; Hoffman, R.

In: Blood, Vol. 74, No. 1, 1989, p. 145-151.

Research output: Contribution to journalArticle

Briddell, RA, Brandt, JE, Straneva, JE, Srour, E & Hoffman, R 1989, 'Characterization of the human burst-forming unit-megakaryocyte', Blood, vol. 74, no. 1, pp. 145-151.
Briddell RA, Brandt JE, Straneva JE, Srour E, Hoffman R. Characterization of the human burst-forming unit-megakaryocyte. Blood. 1989;74(1):145-151.
Briddell, R. A. ; Brandt, J. E. ; Straneva, J. E. ; Srour, Edward ; Hoffman, R. / Characterization of the human burst-forming unit-megakaryocyte. In: Blood. 1989 ; Vol. 74, No. 1. pp. 145-151.
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