Characterization of xenobiotic biotransformation in hepatic, renal and gut tissues of cattle and sheep.

J. B. Watkins, G. S. Smith, D. M. Hallford

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Microsomal and cytosolic preparations of hepatic, renal, ileal and ruminal tissues of cattle and sheep were used to measure oxidative, hydrolative and conjugative biotransformations of 11 xenobiotic substrates. Within species, enzyme activities were generally higher (P less than .05) in hepatic than non-hepatic tissue but, in both species, non-hepatic tissue exhibited considerable capacities for metabolizing certain substrates. Sheep rumen wall (with papillae) was notably high in cytochrome P-450 content (34% of hepatic value), in glutathione conjugation of ethacrynic acid (223% of hepatic activity; P less than .05), and UDP-glucuronidation of estrone (290% of hepatic activity; P less than .05). Sheep differed (P less than .05) from cattle, having lower cytochrome P-450 content in liver and ileum (but not kidney); lower N-demethylase activity in liver, but two- to threefold higher activity in kidney; lower sulfotransferase activity in liver and kidney; and higher glutathione S-transferase activity toward certain substrates. UDP-glucuronidation varied too widely among substrates to afford strong generalization in comparisons among tissues or between species. Non-hepatic tissues in ruminants exhibit considerable capacities for oxidative, hydrolative and conjugative metabolism of xenobiotics. Sheep and cattle differ widely in hepatic and non-hepatic capacities for biotransforming certain xenobiotics.

Original languageEnglish
Pages (from-to)186-195
Number of pages10
JournalJournal of Animal Science
Volume65
Issue number1
StatePublished - Jul 1987
Externally publishedYes

Fingerprint

Xenobiotics
xenobiotics
Biotransformation
biotransformation
Sheep
digestive system
kidneys
Kidney
sheep
liver
Liver
cattle
Uridine Diphosphate
cytochrome P-450
Cytochrome P-450 Enzyme System
N Demethylating Oxidoreductases
ethacrynic acid
Ethacrynic Acid
sulfotransferases
tissues

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Characterization of xenobiotic biotransformation in hepatic, renal and gut tissues of cattle and sheep. / Watkins, J. B.; Smith, G. S.; Hallford, D. M.

In: Journal of Animal Science, Vol. 65, No. 1, 07.1987, p. 186-195.

Research output: Contribution to journalArticle

Watkins, J. B. ; Smith, G. S. ; Hallford, D. M. / Characterization of xenobiotic biotransformation in hepatic, renal and gut tissues of cattle and sheep. In: Journal of Animal Science. 1987 ; Vol. 65, No. 1. pp. 186-195.
@article{0eb5032c34cf478183cc9d5d4a335755,
title = "Characterization of xenobiotic biotransformation in hepatic, renal and gut tissues of cattle and sheep.",
abstract = "Microsomal and cytosolic preparations of hepatic, renal, ileal and ruminal tissues of cattle and sheep were used to measure oxidative, hydrolative and conjugative biotransformations of 11 xenobiotic substrates. Within species, enzyme activities were generally higher (P less than .05) in hepatic than non-hepatic tissue but, in both species, non-hepatic tissue exhibited considerable capacities for metabolizing certain substrates. Sheep rumen wall (with papillae) was notably high in cytochrome P-450 content (34{\%} of hepatic value), in glutathione conjugation of ethacrynic acid (223{\%} of hepatic activity; P less than .05), and UDP-glucuronidation of estrone (290{\%} of hepatic activity; P less than .05). Sheep differed (P less than .05) from cattle, having lower cytochrome P-450 content in liver and ileum (but not kidney); lower N-demethylase activity in liver, but two- to threefold higher activity in kidney; lower sulfotransferase activity in liver and kidney; and higher glutathione S-transferase activity toward certain substrates. UDP-glucuronidation varied too widely among substrates to afford strong generalization in comparisons among tissues or between species. Non-hepatic tissues in ruminants exhibit considerable capacities for oxidative, hydrolative and conjugative metabolism of xenobiotics. Sheep and cattle differ widely in hepatic and non-hepatic capacities for biotransforming certain xenobiotics.",
author = "Watkins, {J. B.} and Smith, {G. S.} and Hallford, {D. M.}",
year = "1987",
month = "7",
language = "English",
volume = "65",
pages = "186--195",
journal = "Journal of Animal Science",
issn = "0021-8812",
publisher = "American Society of Animal Science",
number = "1",

}

TY - JOUR

T1 - Characterization of xenobiotic biotransformation in hepatic, renal and gut tissues of cattle and sheep.

AU - Watkins, J. B.

AU - Smith, G. S.

AU - Hallford, D. M.

PY - 1987/7

Y1 - 1987/7

N2 - Microsomal and cytosolic preparations of hepatic, renal, ileal and ruminal tissues of cattle and sheep were used to measure oxidative, hydrolative and conjugative biotransformations of 11 xenobiotic substrates. Within species, enzyme activities were generally higher (P less than .05) in hepatic than non-hepatic tissue but, in both species, non-hepatic tissue exhibited considerable capacities for metabolizing certain substrates. Sheep rumen wall (with papillae) was notably high in cytochrome P-450 content (34% of hepatic value), in glutathione conjugation of ethacrynic acid (223% of hepatic activity; P less than .05), and UDP-glucuronidation of estrone (290% of hepatic activity; P less than .05). Sheep differed (P less than .05) from cattle, having lower cytochrome P-450 content in liver and ileum (but not kidney); lower N-demethylase activity in liver, but two- to threefold higher activity in kidney; lower sulfotransferase activity in liver and kidney; and higher glutathione S-transferase activity toward certain substrates. UDP-glucuronidation varied too widely among substrates to afford strong generalization in comparisons among tissues or between species. Non-hepatic tissues in ruminants exhibit considerable capacities for oxidative, hydrolative and conjugative metabolism of xenobiotics. Sheep and cattle differ widely in hepatic and non-hepatic capacities for biotransforming certain xenobiotics.

AB - Microsomal and cytosolic preparations of hepatic, renal, ileal and ruminal tissues of cattle and sheep were used to measure oxidative, hydrolative and conjugative biotransformations of 11 xenobiotic substrates. Within species, enzyme activities were generally higher (P less than .05) in hepatic than non-hepatic tissue but, in both species, non-hepatic tissue exhibited considerable capacities for metabolizing certain substrates. Sheep rumen wall (with papillae) was notably high in cytochrome P-450 content (34% of hepatic value), in glutathione conjugation of ethacrynic acid (223% of hepatic activity; P less than .05), and UDP-glucuronidation of estrone (290% of hepatic activity; P less than .05). Sheep differed (P less than .05) from cattle, having lower cytochrome P-450 content in liver and ileum (but not kidney); lower N-demethylase activity in liver, but two- to threefold higher activity in kidney; lower sulfotransferase activity in liver and kidney; and higher glutathione S-transferase activity toward certain substrates. UDP-glucuronidation varied too widely among substrates to afford strong generalization in comparisons among tissues or between species. Non-hepatic tissues in ruminants exhibit considerable capacities for oxidative, hydrolative and conjugative metabolism of xenobiotics. Sheep and cattle differ widely in hepatic and non-hepatic capacities for biotransforming certain xenobiotics.

UR - http://www.scopus.com/inward/record.url?scp=0023376806&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0023376806&partnerID=8YFLogxK

M3 - Article

VL - 65

SP - 186

EP - 195

JO - Journal of Animal Science

JF - Journal of Animal Science

SN - 0021-8812

IS - 1

ER -