Chemical stimulation of the dorsomedial hypothalamus evokes non-shivering thermogenesis in anesthetized rats

Maria V. Zaretskaia, Dmitry Zaretsky, Anantha Shekhar, Joseph A. DiMicco

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Disinhibition of neurons in the dorsomedial hypothalamus (DMH) by microinjection of the GABAA receptor antagonist bicuculline methiodide (BMI) elicits a range of autonomic and endocrine changes resembling those seen in experimental paradigms for emotional stress. Stress in rats is also known to provoke increases in body temperature resulting in part from sympathetically mediated activation of brown fat. The purpose of the present study was to examine the effect of microinjection of BMI into the DMH on core body temperature and temperature of brown fat in rats. In anesthetized preparations, microinjection of BMI 10 pmol/50 nl into the DMH and adjoining posterior hypothalamus elicited marked and rapid increases in temperature in interscapular brown adipose tissue (IBAT) and lesser delayed elevations in rectal temperature. Similar injections into the paraventricular nucleus or ventromedial hypothalamus had no effect on either parameter. Peak increases in IBAT were significantly correlated with both peak increases in core temperature and maximal increases in heart rate that accompanied these changes, and all of these effects were abolished by systemic treatment with propranolol 1 mg/kg. In conscious rats instrumented for telemetry, microinjection of BMI 10 pmol/100 nl into the DMH evoked marked increases in core temperature as well as heart rate, locomotor activity, and plasma ACTH. The increase in core temperature occurred with a delayed time course similar to that seen in anesthetized preparations. These results indicate that activation of neurons in the DMH provokes increases in body temperature resulting in large part from sympathoadrenally-mediated activation of brown fat.

Original languageEnglish
Pages (from-to)113-125
Number of pages13
JournalBrain Research
Volume928
Issue number1-2
DOIs
StatePublished - Feb 22 2002

Fingerprint

Chemical Stimulation
Thermogenesis
Brown Adipose Tissue
Hypothalamus
Microinjections
Temperature
Body Temperature
Heart Rate
Posterior Hypothalamus
GABA-A Receptor Antagonists
Neurons
Telemetry
Paraventricular Hypothalamic Nucleus
Locomotion
Psychological Stress
Propranolol
Adrenocorticotropic Hormone
Injections
bicuculline methiodide

Keywords

  • ACTH
  • Bicuculine
  • Body temperature
  • Brown fat
  • GABA
  • Hypothalamus
  • Propranol

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Chemical stimulation of the dorsomedial hypothalamus evokes non-shivering thermogenesis in anesthetized rats. / Zaretskaia, Maria V.; Zaretsky, Dmitry; Shekhar, Anantha; DiMicco, Joseph A.

In: Brain Research, Vol. 928, No. 1-2, 22.02.2002, p. 113-125.

Research output: Contribution to journalArticle

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