Chemokine regulation of hematopoiesis and the involvement of pertussis toxin-sensitive Gαi proteins

Hal E. Broxmeyer, Byung S. Youn, Chang Kim, Giao Hangoc, Scott Cooper, Charlie Mantel

Research output: Contribution to journalArticle

21 Scopus citations

Abstract

Chemokines have been implicated in regulation of various aspects of hematopoiesis, including negative regulation of the proliferation of immature subsets of myeloid progenitor cells (MPCs), chemotaxis of MPCs, and survival enhancement of MPCs after delayed growth factor addition. Since chemokine receptors are seven-transmembrane-spanning G-protein-linked receptors and the chemotactic effect in vitro of the CXC chemokine SDF-1 is pertussis toxin (PT)-sensitive, implying the involvement of Gαi proteins as mediators of SDF-1-induced chemotaxis, we evaluated the effects of PT on other chemokine actions influencing MPCs. While the in vitro survival-enhancing effects of SDF-1 on GM-CSF and steel factor-dependent mouse bone marrow granulocyte macrophage progenitors (CFU-GM) were pertussis toxin-sensitive, the suppressive effects of the CC chemokine MIP-1α and the CXC chemokine IL-8 on colony formation by GM-CSF and steel factor-sensitive CFU-GM were insensitive to pertussis toxin. These results suggest that not all chemokine-mediated effects on MPCs are necessarily mediated through pertussis toxin-sensitive Gαi proteins.

Original languageEnglish (US)
Pages (from-to)117-128
Number of pages12
JournalAnnals of the New York Academy of Sciences
Volume938
DOIs
StatePublished - Jan 1 2001

Keywords

  • G proteins
  • Hematopoiesis
  • Progenitor cells
  • Proliferation
  • Survival

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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