Chemotaxis and chemokinesis of human prostate tumor cell lines in response to human prostate stromal cell secretory proteins containing a nerve growth factor-like protein

Daniel Djakiew, Beth Pflug, Robert Delsite, Makoto Onoda, John H. Lynch, Gloria Arand, Erik W. Thompson

Research output: Contribution to journalArticle

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Abstract

The migration of three human prostate tumor epithelial cell lines (TSU-pr1, PC-3, DU-145) in response to secreted protein from a human prostate stromal cell line was investigated by using the modified blind-well Boyden chamber assay. Migrated cells were quantified by spectrophotometrically measuring the concentration of crystal violet stain extracted from their nuclei. Cell number was correlated linearly with the concentration of extracted crystal violet stain. All three tumor cell lines showed intrinsic migratory ability in the absence of chemoattractants, such that approximately 1-7% of plated cells migrated across the filter of the Boyden chambers during a 5-h incubation period. Prostate tumor cell migration was significantly enhanced (3-13-fold) in response to stromal cell secretory protein in a dose-dependent manner, whereas bovine serum albumin had no effect on stimulating tumor cell migration. Immunoprecipitation of the stromal cell secreted protein with a nerve growth factor antibody partially and significantly reduced its stimulatory activity for tumor cell migration. A Zigmond-Hirsch matrix assay of tumor cell migration in response to various concentration gradients of stromal cell secreted protein demonstrated both chemotaxis and chemokinesis by all three cell lines. These results are consistent with the stromal cell secretory protein stimulation of chemokinetic tumor cell migration through the capsule of the prostate. Outside of the prostate gland metastasis of tumor cells may occur by chemotaxis to preferential sites containing chemoattractants similar to or related to maintenance factors that can substitute for components of stromal cell secretory protein.

Original languageEnglish (US)
Pages (from-to)1416-1420
Number of pages5
JournalCancer Research
Volume53
Issue number6
StatePublished - Mar 15 1993
Externally publishedYes

Fingerprint

Nerve Growth Factor
Chemotaxis
Stromal Cells
Tumor Cell Line
Prostate
Cell Movement
Gentian Violet
Neoplasms
Proteins
Chemotactic Factors
Coloring Agents
Cell Migration Assays
Cell Line
Aptitude
Bovine Serum Albumin
Immunoprecipitation
Capsules
Cell Count
Epithelial Cells
Maintenance

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Chemotaxis and chemokinesis of human prostate tumor cell lines in response to human prostate stromal cell secretory proteins containing a nerve growth factor-like protein. / Djakiew, Daniel; Pflug, Beth; Delsite, Robert; Onoda, Makoto; Lynch, John H.; Arand, Gloria; Thompson, Erik W.

In: Cancer Research, Vol. 53, No. 6, 15.03.1993, p. 1416-1420.

Research output: Contribution to journalArticle

Djakiew, Daniel ; Pflug, Beth ; Delsite, Robert ; Onoda, Makoto ; Lynch, John H. ; Arand, Gloria ; Thompson, Erik W. / Chemotaxis and chemokinesis of human prostate tumor cell lines in response to human prostate stromal cell secretory proteins containing a nerve growth factor-like protein. In: Cancer Research. 1993 ; Vol. 53, No. 6. pp. 1416-1420.
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