Chlamydia muridarum infection elicits a beta interferon response in murine oviduct epithelial cells dependent on interferon regulatory factor 3 and TRIF

Wilbert Derbigny, Soon Cheol Hong, Micah S. Kerr, M'hamed Temkit, Raymond M. Johnson

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

Chlamydia trachomatis is the most common sexually transmitted bacterial infection in the United States. Utilizing cloned murine oviduct epithelial cell lines, we previously identified Toll-like receptor 2 (TLR2) as the principal epithelial pattern recognition receptor (PRR) for infection-triggered release of the acute inflammatory cytokines interleukin-6 and granulocyte-macrophage colony-stimulating factor. The infected oviduct epithelial cell lines also secreted the immunomodulatory cytokine beta interferon (IFN-β) in a largely MyD88-independent manner. Although TLR3 was the only IFN-β production-capable TLR expressed by the oviduct cell lines, we were not able to determine whether TLR3 was responsible for IFN-β production because the epithelial cells were unresponsive to the TLR3 ligand poly(I-C), and small interfering RNA (siRNA) techniques were ineffective at knocking down TLR3 expression. To further investigate the potential role of TLR3 in the infected epithelial cell secretion of IFN-β, we examined the roles of its downstream signaling molecules TRIF and IFN regulatory factor 3 (IRF-3) using a dominant-negative TRIF molecule and siRNA specific for TRIF and IRF-3. Antagonism of either IRF-3 or TRIF signaling significantly decreased IFN-β production. These data implicate TLR3, or an unknown PRR utilizing TRIF, as the source of IFN-β production by Chlamydia-infected oviduct epithelial cells.

Original languageEnglish
Pages (from-to)1280-1290
Number of pages11
JournalInfection and Immunity
Volume75
Issue number3
DOIs
StatePublished - Mar 2007

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Chlamydia muridarum
Interferon Regulatory Factor-3
Chlamydia Infections
Oviducts
Interferon-beta
Interferons
Epithelial Cells
Pattern Recognition Receptors
Cell Line
Small Interfering RNA
Cytokines
Toll-Like Receptor 2
Chlamydia
Chlamydia trachomatis
Granulocyte-Macrophage Colony-Stimulating Factor
Sexually Transmitted Diseases
Bacterial Infections
Interleukin-6
Ligands
Infection

ASJC Scopus subject areas

  • Immunology

Cite this

Chlamydia muridarum infection elicits a beta interferon response in murine oviduct epithelial cells dependent on interferon regulatory factor 3 and TRIF. / Derbigny, Wilbert; Hong, Soon Cheol; Kerr, Micah S.; Temkit, M'hamed; Johnson, Raymond M.

In: Infection and Immunity, Vol. 75, No. 3, 03.2007, p. 1280-1290.

Research output: Contribution to journalArticle

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