ChlR1 Is Required for Loading Papillomavirus E2 onto Mitotic Chromosomes and Viral Genome Maintenance

Joanna L. Parish, Angela M. Bean, Regina B. Park, Elliot Androphy

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

Autonomously replicating DNA viruses must evade mitotic checkpoints and actively partition their genomes to maintain persistent infection. The E2 protein serves these functions by tethering papillomavirus episomes to mitotic chromosomes; however, the mechanism remains unresolved. We show that E2 binds ChlR1, a DNA helicase that plays a role in sister chromatid cohesion. The E2 mutation W130R fails to bind ChlR1 and correspondingly does not associate with mitotic chromosomes. Viral genomes encoding this E2 mutation are not episomally maintained in cell culture. Notably, E2 W130R binds Brd4, which reportedly acts as a mitotic tether, indicating this interaction is insufficient for E2 association with mitotic chromosomes. RNAi-induced depletion of ChlR1 significantly reduced E2 localization to mitotic chromosomes. These studies provide compelling evidence that ChlR1 association is required for loading the papillomavirus E2 protein onto mitotic chromosomes and represents a kinetochore-independent mechanism for viral genome maintenance and segregation.

Original languageEnglish (US)
Pages (from-to)867-876
Number of pages10
JournalMolecular Cell
Volume24
Issue number6
DOIs
StatePublished - Dec 28 2006
Externally publishedYes

Fingerprint

Viral Genome
Chromosomes
Maintenance
Genome
M Phase Cell Cycle Checkpoints
DNA Helicases
Kinetochores
Mutation
Chromatids
DNA Viruses
RNA Interference
Proteins
Plasmids
Cell Culture Techniques
Infection

Keywords

  • DNA
  • MICROBIO

ASJC Scopus subject areas

  • Molecular Biology

Cite this

ChlR1 Is Required for Loading Papillomavirus E2 onto Mitotic Chromosomes and Viral Genome Maintenance. / Parish, Joanna L.; Bean, Angela M.; Park, Regina B.; Androphy, Elliot.

In: Molecular Cell, Vol. 24, No. 6, 28.12.2006, p. 867-876.

Research output: Contribution to journalArticle

Parish, Joanna L. ; Bean, Angela M. ; Park, Regina B. ; Androphy, Elliot. / ChlR1 Is Required for Loading Papillomavirus E2 onto Mitotic Chromosomes and Viral Genome Maintenance. In: Molecular Cell. 2006 ; Vol. 24, No. 6. pp. 867-876.
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