Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury.

C. H. Chau, D. K. Shum, H. Li, J. Pei, Y. Y. Lui, L. Wirthlin, Y. S. Chan, Xiao-Ming Xu

Research output: Contribution to journalArticle

165 Citations (Scopus)

Abstract

Grafting of Schwann cell-seeded channels into hemisected adult rat thoracic spinal cords has been tested as a strategy to bridge the injured cord. Despite success in guiding axonal growth into the graft, regeneration across the distal graft-host interface into the host spinal cord was limited. We hypothesized that chondroitin sulfate (CS) glycoforms deposited at the gliotic front of the interface constitute a molecular barrier to axonal growth into the host cord. Because CS glycoforms deposited by purified astrocytes in vitro were removable by digestion with chondroitinase ABC, we attempted to achieve likewise by infusion of the enzyme to the host side of the interface. By 1 month post-treatment, significant numbers of regenerating axons crossed an interface that was subdued in macrophage/microglia reaction and decreased in CS-immunopositivity. The axons extended as far into the caudal cord as 5 mm, in contrast to nil in vehicle-infused controls. Fascicular organizations of axon-Schwann cell units within the regenerated tissue cable were better-preserved in enzyme-treated cords than in vehicle-infused controls. We conclude that CS glycoforms deposited during gliosis at the distal graft-host interface could be cleared by the in vivo action of chondroitinase ABC to improve prospects of axonal regeneration into the host spinal cord.

Original languageEnglish (US)
Pages (from-to)194-196
Number of pages3
JournalFASEB Journal
Volume18
Issue number1
StatePublished - 2004
Externally publishedYes

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Chondroitin ABC Lyase
Chondroitin Sulfates
Schwann Cells
Spinal Cord Injuries
Cells
Axons
Spinal Cord
Transplants
Regeneration
Grafts
Gliosis
Microglia
Enzymes
Growth
Astrocytes
Digestion
Thorax
Macrophages
Rats
Cables

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Chau, C. H., Shum, D. K., Li, H., Pei, J., Lui, Y. Y., Wirthlin, L., ... Xu, X-M. (2004). Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury. FASEB Journal, 18(1), 194-196.

Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury. / Chau, C. H.; Shum, D. K.; Li, H.; Pei, J.; Lui, Y. Y.; Wirthlin, L.; Chan, Y. S.; Xu, Xiao-Ming.

In: FASEB Journal, Vol. 18, No. 1, 2004, p. 194-196.

Research output: Contribution to journalArticle

Chau, CH, Shum, DK, Li, H, Pei, J, Lui, YY, Wirthlin, L, Chan, YS & Xu, X-M 2004, 'Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury.', FASEB Journal, vol. 18, no. 1, pp. 194-196.
Chau, C. H. ; Shum, D. K. ; Li, H. ; Pei, J. ; Lui, Y. Y. ; Wirthlin, L. ; Chan, Y. S. ; Xu, Xiao-Ming. / Chondroitinase ABC enhances axonal regrowth through Schwann cell-seeded guidance channels after spinal cord injury. In: FASEB Journal. 2004 ; Vol. 18, No. 1. pp. 194-196.
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