Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins

Thomas A. Milne, Keji Zhao, Jay L. Hess

Research output: Chapter in Book/Report/Conference proceedingChapter

42 Scopus citations

Abstract

Disruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Many of the advances in the understanding of these mechanisms are attributable to the successful development of chromatin immunoprecipitation (ChIP) for in vivo detection of histone modifications as well as chromatin binding regulatory proteins. This is a powerful technique for analyzing histone modifications as well as binding sites for proteins that bind either directly or indirectly to DNA. Here we present two ChIP protocols. The first is particularly useful for identifying histone modifications or binding at specific, known genomic sites. The second, employing serial analysis of gene expression, is particularly powerful for the discovery of previously unidentified sites of modification or binding.

Original languageEnglish (US)
Title of host publicationLeukemia
Subtitle of host publicationMethods and Protocols
EditorsChi Wai Eric So
Pages409-423
Number of pages15
DOIs
StatePublished - Dec 1 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume538
ISSN (Print)1064-3745

Keywords

  • Acetylation
  • ChIP
  • Chromatin immunoprecipitation
  • Histone modification
  • Methylation
  • Quantitative PCR
  • SAGE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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    Milne, T. A., Zhao, K., & Hess, J. L. (2009). Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. In C. W. E. So (Ed.), Leukemia: Methods and Protocols (pp. 409-423). (Methods in Molecular Biology; Vol. 538). https://doi.org/10.1007/978-1-59745-418-6_21