Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins

Thomas A. Milne, Keji Zhao, Jay Hess

Research output: Chapter in Book/Report/Conference proceedingChapter

37 Citations (Scopus)

Abstract

Disruption of epigenetic regulators of transcription is a central mechanism of oncogenesis. Many of the advances in the understanding of these mechanisms are attributable to the successful development of chromatin immunoprecipitation (ChIP) for in vivo detection of histone modifications as well as chromatin binding regulatory proteins. This is a powerful technique for analyzing histone modifications as well as binding sites for proteins that bind either directly or indirectly to DNA. Here we present two ChIP protocols. The first is particularly useful for identifying histone modifications or binding at specific, known genomic sites. The second, employing serial analysis of gene expression, is particularly powerful for the discovery of previously unidentified sites of modification or binding.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages409-423
Number of pages15
Volume538
DOIs
StatePublished - 2009
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume538
ISSN (Print)10643745

Fingerprint

Histone Code
Chromatin Immunoprecipitation
Chromatin
Carrier Proteins
Proteins
Epigenomics
Carcinogenesis
Binding Sites
Gene Expression
DNA

Keywords

  • Acetylation
  • ChIP
  • Chromatin immunoprecipitation
  • Histone modification
  • Methylation
  • Quantitative PCR
  • SAGE

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Milne, T. A., Zhao, K., & Hess, J. (2009). Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. In Methods in Molecular Biology (Vol. 538, pp. 409-423). (Methods in Molecular Biology; Vol. 538). https://doi.org/10.1007/978-1-59745-418-6_21

Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. / Milne, Thomas A.; Zhao, Keji; Hess, Jay.

Methods in Molecular Biology. Vol. 538 2009. p. 409-423 (Methods in Molecular Biology; Vol. 538).

Research output: Chapter in Book/Report/Conference proceedingChapter

Milne, TA, Zhao, K & Hess, J 2009, Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. in Methods in Molecular Biology. vol. 538, Methods in Molecular Biology, vol. 538, pp. 409-423. https://doi.org/10.1007/978-1-59745-418-6_21
Milne TA, Zhao K, Hess J. Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. In Methods in Molecular Biology. Vol. 538. 2009. p. 409-423. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-59745-418-6_21
Milne, Thomas A. ; Zhao, Keji ; Hess, Jay. / Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods in Molecular Biology. Vol. 538 2009. pp. 409-423 (Methods in Molecular Biology).
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