Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes

Hui Wen Liu, Jie Zhang, George F. Heine, Mansi Arora, Hatice Gulcin Ozer, Raghuram Onti-Srinivasan, Kun Huang, Jeffrey D. Parvin

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

SUMOylation of transcription factors and chromatin proteins is in many cases a negative mark that recruits factors that repress gene expression. In this study, we determined the occupancy of Small Ubiquitin-like MOdifier (SUMO)-1 on chromatin in HeLa cells by use of chromatin affinity purification coupled with next-generation sequencing. We found SUMO-1 localization on chromatin was dynamic throughout the cell cycle. Surprisingly, we observed that from G1 through late S phase, but not during mitosis, SUMO-1 marks the chromatin just upstream of the transcription start site on many of the most active housekeeping genes, including genes encoding translation factors and ribosomal subunit proteins. Moreover, we found that SUMO-1 distribution on promoters was correlated with H3K4me3, another general chromatin activation mark. Depletion of SUMO-1 resulted in downregulation of the genes that were marked by SUMO-1 at their promoters during interphase, supporting the concept that the marking of promoters by SUMO-1 is associated with transcriptional activation of genes involved in ribosome biosynthesis and in the protein translation process.

Original languageEnglish (US)
Pages (from-to)10172-10186
Number of pages15
JournalNucleic Acids Research
Volume40
Issue number20
DOIs
StatePublished - Nov 1 2012
Externally publishedYes

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Ubiquitin
Chromatin
Genes
Sumoylation
Ribosome Subunits
Ribosomal Proteins
Transcription Initiation Site
Essential Genes
Interphase
Protein Biosynthesis
Ribosomes
S Phase
HeLa Cells
Mitosis
Transcriptional Activation
Cell Cycle
Transcription Factors
Down-Regulation
Gene Expression
Proteins

ASJC Scopus subject areas

  • Genetics

Cite this

Liu, H. W., Zhang, J., Heine, G. F., Arora, M., Gulcin Ozer, H., Onti-Srinivasan, R., ... Parvin, J. D. (2012). Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes. Nucleic Acids Research, 40(20), 10172-10186. https://doi.org/10.1093/nar/gks819

Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes. / Liu, Hui Wen; Zhang, Jie; Heine, George F.; Arora, Mansi; Gulcin Ozer, Hatice; Onti-Srinivasan, Raghuram; Huang, Kun; Parvin, Jeffrey D.

In: Nucleic Acids Research, Vol. 40, No. 20, 01.11.2012, p. 10172-10186.

Research output: Contribution to journalArticle

Liu, HW, Zhang, J, Heine, GF, Arora, M, Gulcin Ozer, H, Onti-Srinivasan, R, Huang, K & Parvin, JD 2012, 'Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes', Nucleic Acids Research, vol. 40, no. 20, pp. 10172-10186. https://doi.org/10.1093/nar/gks819
Liu HW, Zhang J, Heine GF, Arora M, Gulcin Ozer H, Onti-Srinivasan R et al. Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes. Nucleic Acids Research. 2012 Nov 1;40(20):10172-10186. https://doi.org/10.1093/nar/gks819
Liu, Hui Wen ; Zhang, Jie ; Heine, George F. ; Arora, Mansi ; Gulcin Ozer, Hatice ; Onti-Srinivasan, Raghuram ; Huang, Kun ; Parvin, Jeffrey D. / Chromatin modification by SUMO-1 stimulates the promoters of translation machinery genes. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 20. pp. 10172-10186.
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