Chronic Alcohol Exposure Alters Gene Expression in HepG2 Cells

Sirisha Pochareddy, Howard Edenberg

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Background: The liver is the primary site of alcohol metabolism and is highly vulnerable to injuries due to chronic alcohol abuse. Several molecular mechanisms, including oxidative stress and altered cellular metabolism, have been implicated in the development and progression of alcoholic liver disease. We sought to gain further insight into the molecular pathogenesis by studying the effects of ethanol exposure on the global gene expression in HepG2 cells. Methods: HepG2 cells were cultured in the presence or absence of 75mM ethanol for 9days, with fresh media daily. Global gene expression changes were studied using Affymetrix GeneChip ® Human Exon 1.0 ST Arrays. Gene expression differences were validated for 13 genes by quantitative real-time RT-PCR. To identify biological pathways affected by ethanol treatment, differentially expressed genes were analyzed by Ingenuity Pathway Analysis software. Results: Long-term ethanol exposure altered the expression of 1,093 genes (false discovery rate≤3%); many of these changes were modest. Long-term ethanol exposure affected several pathways, including acute phase response, amino acid metabolism, carbohydrate metabolism, and lipid metabolism. Conclusions: Global measurements of gene expression show that a large number of genes are affected by chronic ethanol, although most show modest effect. These data provide insight into the molecular pathology resulting from extended alcohol exposure.

Original languageEnglish
Pages (from-to)1021-1033
Number of pages13
JournalAlcoholism: Clinical and Experimental Research
Volume36
Issue number6
DOIs
StatePublished - Jun 2012

Fingerprint

Hep G2 Cells
Gene expression
Ethanol
Alcohols
Gene Expression
Genes
Metabolism
Liver
Alcoholic Liver Diseases
Acute-Phase Reaction
Oxidative stress
Molecular Pathology
Carbohydrate Metabolism
Genetic Association Studies
Pathology
Lipid Metabolism
Alcoholism
Real-Time Polymerase Chain Reaction
Exons
Oxidative Stress

Keywords

  • Alcoholism
  • Ethanol
  • Global Gene Expression
  • Liver Cells
  • Pathways

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Psychiatry and Mental health
  • Toxicology

Cite this

Chronic Alcohol Exposure Alters Gene Expression in HepG2 Cells. / Pochareddy, Sirisha; Edenberg, Howard.

In: Alcoholism: Clinical and Experimental Research, Vol. 36, No. 6, 06.2012, p. 1021-1033.

Research output: Contribution to journalArticle

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