Chronic inflammation and protection from acute hepatitis in transgenic mice expressing TNF in endothelial cells

A. Willuweit, G. Sass, A. Schöneberg, U. Eisel, G. Tiegs, Matthias Clauss

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Endothelial activation is an important feature of many inflammatory diseases and has been implicated as the cause of vascular complications in disorders such as diabetes, atherosclerosis, and transplant rejection. One of the most potent activators of the endothelium is TNF, which can also be expressed by endothelial cells, causing a permanent, autocrine stimulatory signal. To establish a model of continuous endothelial activation and to elucidate the role of endothelial derived TNF in vivo, we generated transgenic mice expressing a noncleavable transmembrane form of TNF under the control of the endothelial-specific tie2 promoter. Adult tie2-transmembrane TNF-transgenic mice developed chronic inflammatory pathology in kidney and liver, characterized by perivascular infiltration of mononuclear cells into these organs. Along with the infiltrate, an up-regulation of the adhesion molecules ICAM-1 and VCAM-1, but not E-selectin, in the endothelium was observed. Despite predisposition to chronic inflammation these mice were protected from immune-mediated liver injury in a model of Con A-induced acute hepatitis. Although the blood levels of soluble TNF and IFN-γ were increased in transgenic animals after challenge with Con A, no damage of hepatocytes could be detected, as assessed by the lack of increase in plasma transaminase activities and the absence of TUNEL staining in the liver. We conclude that expression of transmembrane TNF in the endothelium causes continuous endothelial activation, leading to both proinflammatory and protective events.

Original languageEnglish (US)
Pages (from-to)3944-3952
Number of pages9
JournalJournal of Immunology
Volume167
Issue number7
StatePublished - Oct 1 2001
Externally publishedYes

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Transgenic Mice
Hepatitis
Endothelium
Endothelial Cells
Inflammation
Liver
Genetically Modified Animals
E-Selectin
Vascular Cell Adhesion Molecule-1
In Situ Nick-End Labeling
Graft Rejection
Intercellular Adhesion Molecule-1
Transaminases
Blood Vessels
Hepatocytes
Atherosclerosis
Up-Regulation
Pathology
Staining and Labeling
Kidney

ASJC Scopus subject areas

  • Immunology

Cite this

Chronic inflammation and protection from acute hepatitis in transgenic mice expressing TNF in endothelial cells. / Willuweit, A.; Sass, G.; Schöneberg, A.; Eisel, U.; Tiegs, G.; Clauss, Matthias.

In: Journal of Immunology, Vol. 167, No. 7, 01.10.2001, p. 3944-3952.

Research output: Contribution to journalArticle

Willuweit, A, Sass, G, Schöneberg, A, Eisel, U, Tiegs, G & Clauss, M 2001, 'Chronic inflammation and protection from acute hepatitis in transgenic mice expressing TNF in endothelial cells', Journal of Immunology, vol. 167, no. 7, pp. 3944-3952.
Willuweit, A. ; Sass, G. ; Schöneberg, A. ; Eisel, U. ; Tiegs, G. ; Clauss, Matthias. / Chronic inflammation and protection from acute hepatitis in transgenic mice expressing TNF in endothelial cells. In: Journal of Immunology. 2001 ; Vol. 167, No. 7. pp. 3944-3952.
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