Chronic pancreatitis is associated with increased concentrations of epidermal growth factor receptor, transforming growth factor α, and phospholipase Cγ

Murray Korc, H. Friess, Y. Yamanaka, M. S. Kobrin, M. Buchler, H. G. Beger

Research output: Contribution to journalArticle

95 Citations (Scopus)

Abstract

The epidermal growth factor (EGF) receptor is a transmembrane protein that binds EGF and transforming growth factor α (TGF α), and that stimulates phospholipase C γ1 (PLC γ1) activity. In this study the role of the EGF receptor in chronic pancreatitis was studied. By immunohistochemistry, the EGF receptor, TGF α, and PLC γ1 were found to be expressed at high concentrations in pancreatic ductal and acinar cells from chronic pancreatitis patients. Northern blot analysis showed that, by comparison with normal controls, 19 of 27 chronic pancreatitis tissues exhibited a 5.7-fold increase in EGF receptor mRNA concentrations, and 20 of 27 chronic pancreatitis tissues exhibited a sixfold increase in TGF α mRNA concentrations. In situ hybridisation confirmed that overexpression occurred in ductal and acinar cells, and showed that both mRNA moieties colocalised with their respective proteins. These findings suggest that TGF α may act through autocrine and paracrine mechanisms to excessively activate the overexpressed EGF receptor in the two major cell types of the exocrine pancreas, thereby contributing to the pathobiology of this disorder.

Original languageEnglish (US)
Pages (from-to)1468-1473
Number of pages6
JournalGut
Volume35
Issue number10
StatePublished - Oct 1994
Externally publishedYes

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Chronic Pancreatitis
Transforming Growth Factors
Type C Phospholipases
Epidermal Growth Factor Receptor
Acinar Cells
Messenger RNA
Exocrine Pancreas
Epidermal Growth Factor
Northern Blotting
In Situ Hybridization
Proteins
Immunohistochemistry

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Korc, M., Friess, H., Yamanaka, Y., Kobrin, M. S., Buchler, M., & Beger, H. G. (1994). Chronic pancreatitis is associated with increased concentrations of epidermal growth factor receptor, transforming growth factor α, and phospholipase Cγ. Gut, 35(10), 1468-1473.

Chronic pancreatitis is associated with increased concentrations of epidermal growth factor receptor, transforming growth factor α, and phospholipase Cγ. / Korc, Murray; Friess, H.; Yamanaka, Y.; Kobrin, M. S.; Buchler, M.; Beger, H. G.

In: Gut, Vol. 35, No. 10, 10.1994, p. 1468-1473.

Research output: Contribution to journalArticle

Korc, M, Friess, H, Yamanaka, Y, Kobrin, MS, Buchler, M & Beger, HG 1994, 'Chronic pancreatitis is associated with increased concentrations of epidermal growth factor receptor, transforming growth factor α, and phospholipase Cγ', Gut, vol. 35, no. 10, pp. 1468-1473.
Korc, Murray ; Friess, H. ; Yamanaka, Y. ; Kobrin, M. S. ; Buchler, M. ; Beger, H. G. / Chronic pancreatitis is associated with increased concentrations of epidermal growth factor receptor, transforming growth factor α, and phospholipase Cγ. In: Gut. 1994 ; Vol. 35, No. 10. pp. 1468-1473.
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