Chronic posttraumatic epilepsy following neocortical undercut lesion in mice

Xingjie Ping, Xiaoming Jin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Posttraumatic epilepsy (PTE) usually develops in a small percentage of patients of traumatic brain injury after a varying latent period. Modeling this chronic neurological condition in rodents is time consuming and inefficient, which constitutes a significant obstacle in studying its mechanism and discovering novel therapeutics for its prevention and treatment. Partially isolated neocortex, or undercut, is known to induce cortical hyperexcitability and epileptiform activity in vitro, and has been used extensively for studying the neurophysiological mechanism of posttraumatic epileptogenesis. However, whether the undercut lesion in rodents causes chronic epileptic seizures has not been systematically characterized. Here we used a miniature telemetry system to continuously monitor electroencephalography (EEG) in adult C57BL mice for up to 3 months after undercut surgery. We found that 50% of animals developed spontaneous seizures between 16-50 days after injury. The mean seizure duration was 8.9±3.6 seconds, and the average seizure frequency was 0.17±0.17 times per day. There was no progression in seizure frequency and duration over the recording period. Video monitoring revealed behavioral arrests and clonic limb movement during seizure attacks. A pentylenetetrazol (PTZ) test further showed increased seizure susceptibility in the undercut mice. We conclude that undercut lesion in mice is a model of chronic PTE that involves spontaneous epileptic seizures.

Original languageEnglish (US)
Article numbere0158231
JournalPLoS One
Volume11
Issue number6
DOIs
StatePublished - Jun 1 2016

Fingerprint

epilepsy
seizures
lesions (animal)
Epilepsy
Seizures
Pentylenetetrazole
mice
Telemetering
Electroencephalography
Surgery
Brain
Animals
Monitoring
Rodentia
Telemetry
rodents
Neocortex
Inbred C57BL Mouse
neocortex
electroencephalography

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Chronic posttraumatic epilepsy following neocortical undercut lesion in mice. / Ping, Xingjie; Jin, Xiaoming.

In: PLoS One, Vol. 11, No. 6, e0158231, 01.06.2016.

Research output: Contribution to journalArticle

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