Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases

Guang Dai, Till Freudenberger, Petra Zipper, Ariane Melchior, Susanne Grether-Beck, Berit Rabausch, Jens De Groot, Sören Twarock, Helmut Hanenberg, Bernhard Homey, Jean Krutmann, Julia Reifenberger, Jens W. Fischer

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Abstract

Remodeling of the dermal extracellular matrix occurs during photoaging. Here, the effect of repetitive UVB irradiation on dermal hyaluronic acid (HA) was examined. C57/BL6 mice were chronically (182 days) irradiated with UVB, and consecutive skin biopsies were collected during the irradiation period and afterward (300 and 400 days of age). UVB caused marked loss of HA from the papillary dermis and down-regulation of HA synthase 1 (HAS1), HAS2, and HAS3 mRNA expression. In contrast, hyaluronidases (HYAL) 1, HYAL2, and HA receptor CD44 were unchanged. Furthermore, transforming growth factor β-1 (TGF-β1) and TGF-β1-receptor II expression were decreased in UVB-irradiated biopsies, and TGF-β1 strongly induced HAS1 and HAS2 expression in cultured dermal fibroblasts. Therefore, TGF-β1 might be one factor involved in UVB-induced down-regulation of HAS enzymes. In addition, total cell number and the percentage of proliferating fibroblasts in the papillary dermis of UVB-irradiated mice were decreased. Down-regulation of HAS2 by lentiviral overexpression of short hairpin RNA in vitro caused inhibition of HA synthesis, DNA synthesis, and migration of dermal fibroblasts. In conclusion, chronic UVB irradiation induces toss of HA from the dermis, thereby contributing to the quiescent phenotype of dermal fibroblasts.

Original languageEnglish (US)
Pages (from-to)1451-1461
Number of pages11
JournalAmerican Journal of Pathology
Volume171
Issue number5
DOIs
StatePublished - Nov 2007

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ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Dai, G., Freudenberger, T., Zipper, P., Melchior, A., Grether-Beck, S., Rabausch, B., De Groot, J., Twarock, S., Hanenberg, H., Homey, B., Krutmann, J., Reifenberger, J., & Fischer, J. W. (2007). Chronic ultraviolet B irradiation causes loss of hyaluronic acid from mouse dermis because of down-regulation of hyaluronic acid synthases. American Journal of Pathology, 171(5), 1451-1461. https://doi.org/10.2353/ajpath.2007.070136