Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition

C. A. Labarrere, J. W. Hardin, David Haas, G. S. Kassab

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Introduction Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic characteristics. Methods A cross-sectional study of third trimester placentas was conducted to identify immune cell subpopulations in CVUE and MCI (n = 17/group). CVUE was diagnosed with H&E staining and antibody to CD3 in serial sections; and MCI, by the presence of massive infiltration of mononuclear cells in the intervillous spaces. Immune cells, ICAM-1 expression and nuclear factor κB (NF-κB) activation were determined immunohistochemically. Results CVUE and MCI showed similar infiltrates, mainly CD68+ and CD3+ cells. Most cells (>80%) were CD45RB+, and one third were CD45RO+ in both lesions. There were slightly more CD8+ than CD4+ cells in both CVUE and MCI. More than 90% of cells in CVUE and MCI were ICAM-1+ with NFκB nuclear localization. Syncytiotrophoblast ICAM-1 expression was significantly (p <0.001) higher in MCI (mean of 81.0; range of 71.6-86.0) than in CVUE (52.4; 36.4-59.4) or normal placentas (0.2; 0.0-0.6). Both, failure of physiologic transformation of spiral arteries and placental atherosclerosis-like lesions of atherosis were significantly more frequent in MCI than in CVUE or normal placentas (p = 0.044 and p = 0.007, respectively). Discussion These finding suggest that MCI and CVUE have very similar infiltration of immune cells although MCI has more severe placental lesions.

Original languageEnglish (US)
Pages (from-to)681-686
Number of pages6
JournalPlacenta
Volume36
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Intercellular Adhesion Molecule-1
Placenta
Chorionic Villi
Trophoblasts
Third Pregnancy Trimester
Atherosclerosis
Arteries
Cross-Sectional Studies
Staining and Labeling
Antibodies

Keywords

  • Atherosis
  • Failure of physiologic transformation
  • ICAM-1
  • Intervillositis
  • Placenta
  • Pregnancy
  • Spiral arteries
  • Villitis

ASJC Scopus subject areas

  • Obstetrics and Gynecology
  • Reproductive Medicine
  • Developmental Biology
  • Medicine(all)

Cite this

Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition. / Labarrere, C. A.; Hardin, J. W.; Haas, David; Kassab, G. S.

In: Placenta, Vol. 36, No. 6, 01.06.2015, p. 681-686.

Research output: Contribution to journalArticle

Labarrere, C. A. ; Hardin, J. W. ; Haas, David ; Kassab, G. S. / Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition. In: Placenta. 2015 ; Vol. 36, No. 6. pp. 681-686.
@article{f17ada54ae624daf9a2898425281b16e,
title = "Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition",
abstract = "Introduction Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic characteristics. Methods A cross-sectional study of third trimester placentas was conducted to identify immune cell subpopulations in CVUE and MCI (n = 17/group). CVUE was diagnosed with H&E staining and antibody to CD3 in serial sections; and MCI, by the presence of massive infiltration of mononuclear cells in the intervillous spaces. Immune cells, ICAM-1 expression and nuclear factor κB (NF-κB) activation were determined immunohistochemically. Results CVUE and MCI showed similar infiltrates, mainly CD68+ and CD3+ cells. Most cells (>80{\%}) were CD45RB+, and one third were CD45RO+ in both lesions. There were slightly more CD8+ than CD4+ cells in both CVUE and MCI. More than 90{\%} of cells in CVUE and MCI were ICAM-1+ with NFκB nuclear localization. Syncytiotrophoblast ICAM-1 expression was significantly (p <0.001) higher in MCI (mean of 81.0; range of 71.6-86.0) than in CVUE (52.4; 36.4-59.4) or normal placentas (0.2; 0.0-0.6). Both, failure of physiologic transformation of spiral arteries and placental atherosclerosis-like lesions of atherosis were significantly more frequent in MCI than in CVUE or normal placentas (p = 0.044 and p = 0.007, respectively). Discussion These finding suggest that MCI and CVUE have very similar infiltration of immune cells although MCI has more severe placental lesions.",
keywords = "Atherosis, Failure of physiologic transformation, ICAM-1, Intervillositis, Placenta, Pregnancy, Spiral arteries, Villitis",
author = "Labarrere, {C. A.} and Hardin, {J. W.} and David Haas and Kassab, {G. S.}",
year = "2015",
month = "6",
day = "1",
doi = "10.1016/j.placenta.2015.03.008",
language = "English (US)",
volume = "36",
pages = "681--686",
journal = "Placenta",
issn = "0143-4004",
publisher = "W.B. Saunders Ltd",
number = "6",

}

TY - JOUR

T1 - Chronic villitis of unknown etiology and massive chronic intervillositis have similar immune cell composition

AU - Labarrere, C. A.

AU - Hardin, J. W.

AU - Haas, David

AU - Kassab, G. S.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Introduction Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic characteristics. Methods A cross-sectional study of third trimester placentas was conducted to identify immune cell subpopulations in CVUE and MCI (n = 17/group). CVUE was diagnosed with H&E staining and antibody to CD3 in serial sections; and MCI, by the presence of massive infiltration of mononuclear cells in the intervillous spaces. Immune cells, ICAM-1 expression and nuclear factor κB (NF-κB) activation were determined immunohistochemically. Results CVUE and MCI showed similar infiltrates, mainly CD68+ and CD3+ cells. Most cells (>80%) were CD45RB+, and one third were CD45RO+ in both lesions. There were slightly more CD8+ than CD4+ cells in both CVUE and MCI. More than 90% of cells in CVUE and MCI were ICAM-1+ with NFκB nuclear localization. Syncytiotrophoblast ICAM-1 expression was significantly (p <0.001) higher in MCI (mean of 81.0; range of 71.6-86.0) than in CVUE (52.4; 36.4-59.4) or normal placentas (0.2; 0.0-0.6). Both, failure of physiologic transformation of spiral arteries and placental atherosclerosis-like lesions of atherosis were significantly more frequent in MCI than in CVUE or normal placentas (p = 0.044 and p = 0.007, respectively). Discussion These finding suggest that MCI and CVUE have very similar infiltration of immune cells although MCI has more severe placental lesions.

AB - Introduction Chronic villitis of unknown etiology (CVUE) and massive chronic intervillositis (MCI) are placental lesions associated with infiltration of mononuclear cells in the chorionic villi and the intervillous spaces, respectively. It is not well known whether immune cells in CVUE and MCI have similar phenotypic characteristics. Methods A cross-sectional study of third trimester placentas was conducted to identify immune cell subpopulations in CVUE and MCI (n = 17/group). CVUE was diagnosed with H&E staining and antibody to CD3 in serial sections; and MCI, by the presence of massive infiltration of mononuclear cells in the intervillous spaces. Immune cells, ICAM-1 expression and nuclear factor κB (NF-κB) activation were determined immunohistochemically. Results CVUE and MCI showed similar infiltrates, mainly CD68+ and CD3+ cells. Most cells (>80%) were CD45RB+, and one third were CD45RO+ in both lesions. There were slightly more CD8+ than CD4+ cells in both CVUE and MCI. More than 90% of cells in CVUE and MCI were ICAM-1+ with NFκB nuclear localization. Syncytiotrophoblast ICAM-1 expression was significantly (p <0.001) higher in MCI (mean of 81.0; range of 71.6-86.0) than in CVUE (52.4; 36.4-59.4) or normal placentas (0.2; 0.0-0.6). Both, failure of physiologic transformation of spiral arteries and placental atherosclerosis-like lesions of atherosis were significantly more frequent in MCI than in CVUE or normal placentas (p = 0.044 and p = 0.007, respectively). Discussion These finding suggest that MCI and CVUE have very similar infiltration of immune cells although MCI has more severe placental lesions.

KW - Atherosis

KW - Failure of physiologic transformation

KW - ICAM-1

KW - Intervillositis

KW - Placenta

KW - Pregnancy

KW - Spiral arteries

KW - Villitis

UR - http://www.scopus.com/inward/record.url?scp=84929653512&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84929653512&partnerID=8YFLogxK

U2 - 10.1016/j.placenta.2015.03.008

DO - 10.1016/j.placenta.2015.03.008

M3 - Article

C2 - 25911290

AN - SCOPUS:84929653512

VL - 36

SP - 681

EP - 686

JO - Placenta

JF - Placenta

SN - 0143-4004

IS - 6

ER -