Chronically elevated levels of short-chain fatty acids induce T Cell-mediated ureteritis and hydronephrosis

Jeongho Park, Craig J. Goergen, Harm HogenEsch, Chang H. Kim

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Short-chain fatty acids (SCFAs) are major products of gut microbial fermentation and profoundly affect host health and disease. SCFAs generate IL-10+ regulatory T cells, which may promote immune tolerance. However, SCFAs can also induce Th1 and Th17 cells upon immunological challenges and, therefore, also have the potential to induce inflammatory responses. Because of the seemingly paradoxical SCFA activities in regulating T cells, we investigated, in depth, the impact of elevated SCFA levels on T cells and tissue inflammation in mice. Orally administered SCFAs induced effector (Th1 and Th17) and regulatory T cells in ureter and kidney tissues, and they induced T cell-mediated ureteritis, leading to kidney hydronephrosis (hereafter called acetate-induced renal disease, or C2RD). Kidney hydronephrosis in C2RD was caused by ureteral obstruction, which was, in turn, induced by SCFA-induced inflammation in the ureteropelvic junction and proximal ureter. Oral administration of all major SCFAs, such as acetate, propionate, and butyrate, induced the disease. We found that C2RD development is dependent on mammalian target of rapamycin activation, T cell-derived inflammatory cytokines such as IFN-γ and IL-17, and gut microbiota. Young or male animals were more susceptible than old or female animals, respectively. However, SCFA receptor (GPR41 or GPR43) deficiency did not affect C2RD development. Thus, SCFAs, when systemically administered at levels higher than physiological levels, cause dysregulated T cell responses and tissue inflammation in the renal system. The results provide insights into the immunological and pathological effects of chronically elevated SCFAs.

Original languageEnglish (US)
Pages (from-to)2388-2400
Number of pages13
JournalJournal of Immunology
Volume196
Issue number5
DOIs
StatePublished - Mar 1 2016
Externally publishedYes

Fingerprint

Volatile Fatty Acids
Hydronephrosis
T-Lymphocytes
Kidney
Regulatory T-Lymphocytes
Ureter
Inflammation
Acetates
Th17 Cells
Ureteral Obstruction
Immune Tolerance
Th1 Cells
Interleukin-17
Butyrates
Propionates
Sirolimus
Interleukin-10
Fermentation
Oral Administration
Cytokines

ASJC Scopus subject areas

  • Immunology

Cite this

Chronically elevated levels of short-chain fatty acids induce T Cell-mediated ureteritis and hydronephrosis. / Park, Jeongho; Goergen, Craig J.; HogenEsch, Harm; Kim, Chang H.

In: Journal of Immunology, Vol. 196, No. 5, 01.03.2016, p. 2388-2400.

Research output: Contribution to journalArticle

Park, Jeongho ; Goergen, Craig J. ; HogenEsch, Harm ; Kim, Chang H. / Chronically elevated levels of short-chain fatty acids induce T Cell-mediated ureteritis and hydronephrosis. In: Journal of Immunology. 2016 ; Vol. 196, No. 5. pp. 2388-2400.
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