Cigarette smoke exposure inhibits contact hypersensitivity via the generation of platelet-activating factor agonists

Ravi P. Sahu, Irina Petrache, Mary J. Van Demark, Badri M. Rashid, Jesus A. Ocana, Yuxuan Tang, Qiaofang Yi, Matthew J. Turner, Raymond Konger, Jeffrey Travers

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Previous studies have established that pro-oxidative stressors suppress host immunity because of their ability to generate oxidized lipids with platelet-activating factor receptor (PAF-R) agonist activity. Although exposure to the pro-oxidative stressor cigarette smoke (CS) is known to exert immunomodulatory effects, little is known regarding the role of PAF in these events. The current studies sought to determine the role of PAF-R signaling in CS-mediated immunomodulatory effects. We demonstrate that CS exposure induces the generation of a transient PAF-R agonistic activity in the blood of mice. CS exposure inhibits contact hypersensitivity in a PAF-R-dependent manner as PAF-R-deficient mice were resistant to these effects. Blocking PAF-R agonist production either by systemic antioxidants or treatment with serum PAF-acetyl hydrolase enzyme blocked both the CS-mediated generation of PAF-R agonists and PAF-R-dependent inhibition of contact hypersensitivity (CHS) reactions, indicating a role for oxidized glycerophosphocholines with PAF-R agonistic activity in this process. In addition, cyclooxygenase-2 inhibition did not block PAFR agonist production but prevented CS-induced inhibition of CHS. This suggests that cyclooxygenase-2 acts downstream of the PAF-R in mediating CS-induced systemic immunosuppression. Moreover, CS exposure induced a significant increase in the expression of the regulatory T cell reporter gene in Foxp3EGFP mice but not in Foxp3EGFP mice on a PAF-R-deficient background. Finally, regulatory T cell depletion via anti-CD25 Abs blocked CS-mediated inhibition of CHS, indicating the potential involvement of regulatory T cells in CS-mediated systemic immunosuppression. These studies provide the first evidence, to our knowledge, that the pro-oxidative stressor CS can modulate cutaneous immunity via the generation of PAF-R agonists produced through lipid oxidation.

Original languageEnglish
Pages (from-to)2447-2454
Number of pages8
JournalJournal of Immunology
Volume190
Issue number5
DOIs
StatePublished - Mar 1 2013

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Platelet Activating Factor
Contact Dermatitis
Smoke
Tobacco Products
Regulatory T-Lymphocytes
Cyclooxygenase 2
Immunosuppression
platelet activating factor receptor
Immunity
Lipids
Hydrolases
Reporter Genes
Antioxidants

ASJC Scopus subject areas

  • Immunology

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Cigarette smoke exposure inhibits contact hypersensitivity via the generation of platelet-activating factor agonists. / Sahu, Ravi P.; Petrache, Irina; Van Demark, Mary J.; Rashid, Badri M.; Ocana, Jesus A.; Tang, Yuxuan; Yi, Qiaofang; Turner, Matthew J.; Konger, Raymond; Travers, Jeffrey.

In: Journal of Immunology, Vol. 190, No. 5, 01.03.2013, p. 2447-2454.

Research output: Contribution to journalArticle

Sahu, Ravi P. ; Petrache, Irina ; Van Demark, Mary J. ; Rashid, Badri M. ; Ocana, Jesus A. ; Tang, Yuxuan ; Yi, Qiaofang ; Turner, Matthew J. ; Konger, Raymond ; Travers, Jeffrey. / Cigarette smoke exposure inhibits contact hypersensitivity via the generation of platelet-activating factor agonists. In: Journal of Immunology. 2013 ; Vol. 190, No. 5. pp. 2447-2454.
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