CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions

Athena W. Wong, W. June Brickey, Debra J. Taxman, Hendrick W. van Deventer, William Reed, Jian Xin Gao, Pan Zheng, Yang Liu, Ping Li, Janice Blum, Karen P. McKinnon, Jenny P Y Ting

Research output: Contribution to journalArticle

118 Citations (Scopus)

Abstract

The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs. Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.

Original languageEnglish
Pages (from-to)891-898
Number of pages8
JournalNature Immunology
Volume4
Issue number9
DOIs
StatePublished - Sep 1 2003

Fingerprint

Cell Communication
Dendritic Cells
Major Histocompatibility Complex
T-Lymphocytes
Semaphorins
MHC Class II Genes
Peptides
Antigen Presentation
RNA Interference
Oligonucleotide Array Sequence Analysis
Small Interfering RNA
Nucleotides
MHC class II transactivator protein
plexin
Antigens
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Wong, A. W., Brickey, W. J., Taxman, D. J., van Deventer, H. W., Reed, W., Gao, J. X., ... Ting, J. P. Y. (2003). CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. Nature Immunology, 4(9), 891-898. https://doi.org/10.1038/ni960

CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. / Wong, Athena W.; Brickey, W. June; Taxman, Debra J.; van Deventer, Hendrick W.; Reed, William; Gao, Jian Xin; Zheng, Pan; Liu, Yang; Li, Ping; Blum, Janice; McKinnon, Karen P.; Ting, Jenny P Y.

In: Nature Immunology, Vol. 4, No. 9, 01.09.2003, p. 891-898.

Research output: Contribution to journalArticle

Wong, AW, Brickey, WJ, Taxman, DJ, van Deventer, HW, Reed, W, Gao, JX, Zheng, P, Liu, Y, Li, P, Blum, J, McKinnon, KP & Ting, JPY 2003, 'CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions', Nature Immunology, vol. 4, no. 9, pp. 891-898. https://doi.org/10.1038/ni960
Wong AW, Brickey WJ, Taxman DJ, van Deventer HW, Reed W, Gao JX et al. CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. Nature Immunology. 2003 Sep 1;4(9):891-898. https://doi.org/10.1038/ni960
Wong, Athena W. ; Brickey, W. June ; Taxman, Debra J. ; van Deventer, Hendrick W. ; Reed, William ; Gao, Jian Xin ; Zheng, Pan ; Liu, Yang ; Li, Ping ; Blum, Janice ; McKinnon, Karen P. ; Ting, Jenny P Y. / CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. In: Nature Immunology. 2003 ; Vol. 4, No. 9. pp. 891-898.
@article{e7f2149aac77469b89726e3e9c3a042b,
title = "CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions",
abstract = "The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs. Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.",
author = "Wong, {Athena W.} and Brickey, {W. June} and Taxman, {Debra J.} and {van Deventer}, {Hendrick W.} and William Reed and Gao, {Jian Xin} and Pan Zheng and Yang Liu and Ping Li and Janice Blum and McKinnon, {Karen P.} and Ting, {Jenny P Y}",
year = "2003",
month = "9",
day = "1",
doi = "10.1038/ni960",
language = "English",
volume = "4",
pages = "891--898",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "Nature Publishing Group",
number = "9",

}

TY - JOUR

T1 - CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions

AU - Wong, Athena W.

AU - Brickey, W. June

AU - Taxman, Debra J.

AU - van Deventer, Hendrick W.

AU - Reed, William

AU - Gao, Jian Xin

AU - Zheng, Pan

AU - Liu, Yang

AU - Li, Ping

AU - Blum, Janice

AU - McKinnon, Karen P.

AU - Ting, Jenny P Y

PY - 2003/9/1

Y1 - 2003/9/1

N2 - The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs. Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.

AB - The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs. Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.

UR - http://www.scopus.com/inward/record.url?scp=0041732281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0041732281&partnerID=8YFLogxK

U2 - 10.1038/ni960

DO - 10.1038/ni960

M3 - Article

C2 - 12910265

AN - SCOPUS:0041732281

VL - 4

SP - 891

EP - 898

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

IS - 9

ER -