CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions

Athena W. Wong, W. June Brickey, Debra J. Taxman, Hendrick W. van Deventer, William Reed, Jian Xin Gao, Pan Zheng, Yang Liu, Ping Li, Janice S. Blum, Karen P. McKinnon, Jenny P.Y. Ting

Research output: Contribution to journalArticle

119 Scopus citations

Abstract

The major histocompatibility complex (MHC) class II transactivator (CIITA) is the 'master coactivator' of MHC class II genes. To identify new targets of CIITA, we analyzed cDNA microarrays of dendritic cells (DCs) from CIITA-deficient, MHC class II-deficient and control mice. We found the semaphorin receptor plexin-A1 was expressed in DCs, but not in other immune cells, and was strongly induced by CIITA. RNA interference by short hairpin RNA specific for plexin-A1, but not a single-nucleotide mutant, greatly reduced plexin-A1 expression and T cell stimulation by protein- or peptide-antigen-pulsed DCs. Plexin-A1 is not required for peptide binding to MHC. These data indicate involvement of plexin-A1 in T cell-DC interactions but not antigen processing or binding.

Original languageEnglish (US)
Pages (from-to)891-898
Number of pages8
JournalNature Immunology
Volume4
Issue number9
DOIs
StatePublished - Sep 1 2003

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Wong, A. W., Brickey, W. J., Taxman, D. J., van Deventer, H. W., Reed, W., Gao, J. X., Zheng, P., Liu, Y., Li, P., Blum, J. S., McKinnon, K. P., & Ting, J. P. Y. (2003). CIITA-regulated plexin-A1 affects T-cell-dendritic cell interactions. Nature Immunology, 4(9), 891-898. https://doi.org/10.1038/ni960