Circulating endothelial cells in pulmonary hypertension

Todd M. Bull, Heiko Golpon, Robert P. Hebbel, Anna Solovey, Carlyne D. Cool, Rubin M. Tuder, Mark W. Geraci, Norbert F. Voelkel

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

The pulmonary endothelium plays a significant role in the pathobiology of Primary Pulmonary Hypertension. A number of diseases, related by a history of vascular injury, are associated with increased numbers of circulating endothelial cells (CECs). We hypothesized that patients with pulmonary hypertension would also have an increased number of circulating endothelial cells due to the high pressures and increased shear stress present within the pulmonary vasculature. We isolated the CECs from 14 patients with pulmonary hypertension, (5 primary and 11 secondary) and compared them to the cells from 12 normal controls. There was a significant increase in the number of CECs in peripheral blood in patients with both PPH and secondary pulmonary hypertension (SPH) when compared to normal volunteers (33.1 +/- 1.9 {PPH} and 27.2 +/- 6.9 {SPH} vs. 3.5 +/- 1.3 {controls}, p <0.001). The number of circulating endothelial cells in the patient's peripheral blood correlated significantly with the systolic, diastolic and mean pulmonary artery pressures of the individual. Approximately 50% of the CECs from patients with pulmonary hypertension expressed CD36, a marker of microvascular origin and 25% expressed E-selectin, a marker of endothelial cell activation. Although the origin of the CECs in patients with PH requires further investigation, one possible source is the pulmonary vasculature, and in patients with plexogenic pulmonary hypertension, the plexiform lesions. CECs may provide a non-invasive mean of accessing cells important to the pathobiology of severe pulmonary hypertension.

Original languageEnglish (US)
Pages (from-to)698-703
Number of pages6
JournalThrombosis and Haemostasis
Volume90
Issue number4
StatePublished - Oct 1 2003
Externally publishedYes

Fingerprint

Pulmonary Hypertension
Endothelial Cells
Lung
Pressure
E-Selectin
Vascular System Injuries
Pulmonary Artery
Endothelium
Healthy Volunteers

Keywords

  • Angiogenesis
  • Circulating endothelial cells
  • Plexiform lesion
  • Primary pulmonary hypertension

ASJC Scopus subject areas

  • Hematology

Cite this

Bull, T. M., Golpon, H., Hebbel, R. P., Solovey, A., Cool, C. D., Tuder, R. M., ... Voelkel, N. F. (2003). Circulating endothelial cells in pulmonary hypertension. Thrombosis and Haemostasis, 90(4), 698-703.

Circulating endothelial cells in pulmonary hypertension. / Bull, Todd M.; Golpon, Heiko; Hebbel, Robert P.; Solovey, Anna; Cool, Carlyne D.; Tuder, Rubin M.; Geraci, Mark W.; Voelkel, Norbert F.

In: Thrombosis and Haemostasis, Vol. 90, No. 4, 01.10.2003, p. 698-703.

Research output: Contribution to journalArticle

Bull, TM, Golpon, H, Hebbel, RP, Solovey, A, Cool, CD, Tuder, RM, Geraci, MW & Voelkel, NF 2003, 'Circulating endothelial cells in pulmonary hypertension', Thrombosis and Haemostasis, vol. 90, no. 4, pp. 698-703.
Bull TM, Golpon H, Hebbel RP, Solovey A, Cool CD, Tuder RM et al. Circulating endothelial cells in pulmonary hypertension. Thrombosis and Haemostasis. 2003 Oct 1;90(4):698-703.
Bull, Todd M. ; Golpon, Heiko ; Hebbel, Robert P. ; Solovey, Anna ; Cool, Carlyne D. ; Tuder, Rubin M. ; Geraci, Mark W. ; Voelkel, Norbert F. / Circulating endothelial cells in pulmonary hypertension. In: Thrombosis and Haemostasis. 2003 ; Vol. 90, No. 4. pp. 698-703.
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