Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis

Jannik Helweg-Larsen, Chao Hung Lee, Shaoling Jin, John Yi Chung Hsueh, Thomas L. Benfield, Jan Hansen, Jens D. Lundgren, Bettina Lundgren

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48 Scopus citations

Abstract

Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23%) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60%) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53%) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46%) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17%) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23% of PCP patients being infected with more than one P. carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP.

Original languageEnglish (US)
Pages (from-to)451-459
Number of pages9
JournalAIDS
Volume15
Issue number4
DOIs
StatePublished - Mar 9 2001

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Keywords

  • Genotyping
  • HIV/AIDS
  • Internal transcribed spacer region
  • Pneumocystis carinii pneumonia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Helweg-Larsen, J., Lee, C. H., Jin, S., Hsueh, J. Y. C., Benfield, T. L., Hansen, J., Lundgren, J. D., & Lundgren, B. (2001). Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis. AIDS, 15(4), 451-459. https://doi.org/10.1097/00002030-200103090-00003