Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis

Jannik Helweg-Larsen, Chao-Hung Lee, Shaoling Jin, John Yi Chung Hsueh, Thomas L. Benfield, Jan Hansen, Jens D. Lundgren, Bettina Lundgren

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23%) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60%) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53%) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46%) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17%) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23% of PCP patients being infected with more than one P. carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP.

Original languageEnglish
Pages (from-to)451-459
Number of pages9
JournalAIDS
Volume15
Issue number4
DOIs
StatePublished - Mar 9 2001

Fingerprint

Pneumocystis carinii
rRNA Genes
Pneumocystis Pneumonia
Genotype
Bronchoscopy
rRNA Operon
Infection
Bronchoalveolar Lavage
Genetic Markers
Sequence Analysis
Pneumonia
Acquired Immunodeficiency Syndrome
Cohort Studies
Prospective Studies

Keywords

  • Genotyping
  • HIV/AIDS
  • Internal transcribed spacer region
  • Pneumocystis carinii pneumonia

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Helweg-Larsen, J., Lee, C-H., Jin, S., Hsueh, J. Y. C., Benfield, T. L., Hansen, J., ... Lundgren, B. (2001). Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis. AIDS, 15(4), 451-459. https://doi.org/10.1097/00002030-200103090-00003

Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis. / Helweg-Larsen, Jannik; Lee, Chao-Hung; Jin, Shaoling; Hsueh, John Yi Chung; Benfield, Thomas L.; Hansen, Jan; Lundgren, Jens D.; Lundgren, Bettina.

In: AIDS, Vol. 15, No. 4, 09.03.2001, p. 451-459.

Research output: Contribution to journalArticle

Helweg-Larsen, J, Lee, C-H, Jin, S, Hsueh, JYC, Benfield, TL, Hansen, J, Lundgren, JD & Lundgren, B 2001, 'Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis', AIDS, vol. 15, no. 4, pp. 451-459. https://doi.org/10.1097/00002030-200103090-00003
Helweg-Larsen, Jannik ; Lee, Chao-Hung ; Jin, Shaoling ; Hsueh, John Yi Chung ; Benfield, Thomas L. ; Hansen, Jan ; Lundgren, Jens D. ; Lundgren, Bettina. / Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis. In: AIDS. 2001 ; Vol. 15, No. 4. pp. 451-459.
@article{5be9827577524a3583a2efb300e65f9c,
title = "Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis",
abstract = "Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23{\%}) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60{\%}) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53{\%}) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46{\%}) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17{\%}) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23{\%} of PCP patients being infected with more than one P. carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP.",
keywords = "Genotyping, HIV/AIDS, Internal transcribed spacer region, Pneumocystis carinii pneumonia",
author = "Jannik Helweg-Larsen and Chao-Hung Lee and Shaoling Jin and Hsueh, {John Yi Chung} and Benfield, {Thomas L.} and Jan Hansen and Lundgren, {Jens D.} and Bettina Lundgren",
year = "2001",
month = "3",
day = "9",
doi = "10.1097/00002030-200103090-00003",
language = "English",
volume = "15",
pages = "451--459",
journal = "AIDS",
issn = "0269-9370",
publisher = "Lippincott Williams and Wilkins",
number = "4",

}

TY - JOUR

T1 - Clinical correlation of variations in the internal transcribed spacer regions of rRNA genes in Pneumocystis carinii f.sp. hominis

AU - Helweg-Larsen, Jannik

AU - Lee, Chao-Hung

AU - Jin, Shaoling

AU - Hsueh, John Yi Chung

AU - Benfield, Thomas L.

AU - Hansen, Jan

AU - Lundgren, Jens D.

AU - Lundgren, Bettina

PY - 2001/3/9

Y1 - 2001/3/9

N2 - Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23%) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60%) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53%) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46%) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17%) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23% of PCP patients being infected with more than one P. carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP.

AB - Objectives: To analyse the importance of sequence variations in the internal transcribed spacer (ITS) regions 1 and 2 of the nuclear rRNA operon in AIDS patients with Pneumocystis carinii pneumonia (PCP). Design and methods: ITS 1 and 2 genotypes were determined in 162 bronchoalveolar lavage samples from 130 patients participating in a prospective cohort study of PCP. Results: A total of 49 different ITS genotypes were detected. ITS genotype was not associated with the clinical severity or outcome of PCP. In 37 of 162 (23%) samples infection with two or more genotypes was observed. A genotype switch was detected in six of 10 patients (60%) with recurrent episodes of PCP. However, genotype changes were also seen in 10 of 19 patients (53%) who had repeated bronchoscopies within the same episode of PCP. The same ITS type was observed twice in 13 (46%) of the 28 patients with repeat bronchoscopies during single or recurrent episodes of pneumonia, but in only 14 of 81 (17%) randomly selected pairs (P < 0.01). Conclusion: Although the detection of ITS genotypes is not a random event, changes in genotype can be detected in a single episode of disease, with 23% of PCP patients being infected with more than one P. carinii genotype, thus complicating the use of this locus as a genetic marker to separate new infection from the reactivation of latent infection. ITS genotypes are not associated with the clinical severity of PCP.

KW - Genotyping

KW - HIV/AIDS

KW - Internal transcribed spacer region

KW - Pneumocystis carinii pneumonia

UR - http://www.scopus.com/inward/record.url?scp=0035831124&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035831124&partnerID=8YFLogxK

U2 - 10.1097/00002030-200103090-00003

DO - 10.1097/00002030-200103090-00003

M3 - Article

C2 - 11242141

AN - SCOPUS:0035831124

VL - 15

SP - 451

EP - 459

JO - AIDS

JF - AIDS

SN - 0269-9370

IS - 4

ER -