Clinical retroviral vector production: Step filtration using clinically approved filters improves titers

L. Reeves, K. Cornetta

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Production of retroviral vectors for clinical use requires removal of cells and cellular debris. We combined a series of filters of decreasing pore size using commercially available blood banking filters approved for clinical use. The collection bag and filters can be connected to create a sterile, closed system using clinically approved tubing and sealing systems. Even when challenged with a large number of vector producer cells (2.38 x 109 cells), no viable cells are passed through the system. The step filtration system developed minimizes the titer reduction associated with filtration, provides rapid flow rates, and was cost effective when filtering volumes in excess of 2 liters.

Original languageEnglish (US)
Pages (from-to)1993-1998
Number of pages6
JournalGene Therapy
Volume7
Issue number23
DOIs
StatePublished - Dec 1 2000

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Keywords

  • Filtration
  • Retroviral vector
  • Vector titer

ASJC Scopus subject areas

  • Genetics

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