Abstract
To characterize the four common Qa-1 allelic products, we examined in detail the CTL-defined determinants encoded by Qa-1. In previous studies with anti-Qa-1 CTL and alloantisera, investigators have described antigenic determinants present on Qa-1(a) and Qa-1(b) antigens, but they have defined Qa-1(c) and Qa-1(d) exclusively by their cross-reactivity with Qa-1(a) and/or Qa-1(b) determinants. To delineate further the CTL-defined determinants encoded by Qa-1(d), we generated CTL clones with Qa-1(d) specificity and demonstrated that the Qa-1(d) molecule expressed determinants that were not detected on Qa-1(a), Qa-1(b), or Qa-1(c) target cells. Other CTL clones derived from anti-Qa-1(d) MLC recognized new antigenic determinants on Qa-1(c) that cross-reacted with Qa-1(d). Each of the four common Qa-1 phenotypes was shown to exhibit unique antigenic determinants. In addition, Qa-1(d) and Qa-1(a) and Qa-1(d) anti-Qa-1(b) CTL confirmed extensive cross-reactivity among these Qa-1 alloantigens. Analysis of CTL from these four immunizations also resulted in the isolation of Qa-1(a)-specific and Qa-1(d)-specific CTL clones that cross-reacted with H-2D(f) and H-2K(s), respectively.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 383-388 |
| Number of pages | 6 |
| Journal | Journal of Immunology |
| Volume | 136 |
| Issue number | 2 |
| State | Published - 1986 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Immunology
Cite this
Clonal analysis of the anti-Qa-1 cytotoxic T lymphocyte repertoire : Definition of the Qa-1(d) and Qa-1(c) alloantigens and cross-reactivity with H-21. / Aldrich, Carla; Jenkins, R. N.; Rich, R. R.
In: Journal of Immunology, Vol. 136, No. 2, 1986, p. 383-388.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Clonal analysis of the anti-Qa-1 cytotoxic T lymphocyte repertoire
T2 - Definition of the Qa-1(d) and Qa-1(c) alloantigens and cross-reactivity with H-21
AU - Aldrich, Carla
AU - Jenkins, R. N.
AU - Rich, R. R.
PY - 1986
Y1 - 1986
N2 - To characterize the four common Qa-1 allelic products, we examined in detail the CTL-defined determinants encoded by Qa-1. In previous studies with anti-Qa-1 CTL and alloantisera, investigators have described antigenic determinants present on Qa-1(a) and Qa-1(b) antigens, but they have defined Qa-1(c) and Qa-1(d) exclusively by their cross-reactivity with Qa-1(a) and/or Qa-1(b) determinants. To delineate further the CTL-defined determinants encoded by Qa-1(d), we generated CTL clones with Qa-1(d) specificity and demonstrated that the Qa-1(d) molecule expressed determinants that were not detected on Qa-1(a), Qa-1(b), or Qa-1(c) target cells. Other CTL clones derived from anti-Qa-1(d) MLC recognized new antigenic determinants on Qa-1(c) that cross-reacted with Qa-1(d). Each of the four common Qa-1 phenotypes was shown to exhibit unique antigenic determinants. In addition, Qa-1(d) and Qa-1(a) and Qa-1(d) anti-Qa-1(b) CTL confirmed extensive cross-reactivity among these Qa-1 alloantigens. Analysis of CTL from these four immunizations also resulted in the isolation of Qa-1(a)-specific and Qa-1(d)-specific CTL clones that cross-reacted with H-2D(f) and H-2K(s), respectively.
AB - To characterize the four common Qa-1 allelic products, we examined in detail the CTL-defined determinants encoded by Qa-1. In previous studies with anti-Qa-1 CTL and alloantisera, investigators have described antigenic determinants present on Qa-1(a) and Qa-1(b) antigens, but they have defined Qa-1(c) and Qa-1(d) exclusively by their cross-reactivity with Qa-1(a) and/or Qa-1(b) determinants. To delineate further the CTL-defined determinants encoded by Qa-1(d), we generated CTL clones with Qa-1(d) specificity and demonstrated that the Qa-1(d) molecule expressed determinants that were not detected on Qa-1(a), Qa-1(b), or Qa-1(c) target cells. Other CTL clones derived from anti-Qa-1(d) MLC recognized new antigenic determinants on Qa-1(c) that cross-reacted with Qa-1(d). Each of the four common Qa-1 phenotypes was shown to exhibit unique antigenic determinants. In addition, Qa-1(d) and Qa-1(a) and Qa-1(d) anti-Qa-1(b) CTL confirmed extensive cross-reactivity among these Qa-1 alloantigens. Analysis of CTL from these four immunizations also resulted in the isolation of Qa-1(a)-specific and Qa-1(d)-specific CTL clones that cross-reacted with H-2D(f) and H-2K(s), respectively.
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UR - http://www.scopus.com/inward/citedby.url?scp=0022631274&partnerID=8YFLogxK
M3 - Article
C2 - 2416805
AN - SCOPUS:0022631274
VL - 136
SP - 383
EP - 388
JO - Journal of Immunology
JF - Journal of Immunology
SN - 0022-1767
IS - 2
ER -