Cloning and characterization of a cDNA encoding murine coagulation factor X

Zhong Liang, Adrian Cooper, Melanie E. DeFord, Peter Carmeliet, Desire Collen, Francis J. Castellino, Elliot Rosen

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The cDNA encoding murine coagulation factor X (fX) was isolated and reconstructed from a λZap cDNA library generated from murine liver mRNA. The cDNA contains 1486 bases starting at the 5'-translation initiation codon. It includes an open reading frame of 1443 nucleotides, followed by an 18 residue 3' nontranslated sequence downstream of the first stop codon, and a 3' poly(A) tail. The translation product is composed of a 40-amino acid signal/propeptide region followed by a 441-residue mature protein. The latter is highly homologous to that of human and rat fX. All protein domains of human and rat fX are strictly conserved in mouse fX. The cDNA coding for mouse fX has been expressed in human embryonic kidney 293 cells and generates fX activity measured in a clotting assay using human fX-deficient plasma.

Original languageEnglish (US)
Pages (from-to)87-91
Number of pages5
JournalThrombosis and Haemostasis
Volume80
Issue number1
StatePublished - Jul 1998
Externally publishedYes

Fingerprint

Factor X
Organism Cloning
Complementary DNA
Messenger RNA
Initiator Codon
Terminator Codon
Gene Library
Open Reading Frames
Nucleotides
Kidney
Amino Acids
Liver

ASJC Scopus subject areas

  • Hematology

Cite this

Liang, Z., Cooper, A., DeFord, M. E., Carmeliet, P., Collen, D., Castellino, F. J., & Rosen, E. (1998). Cloning and characterization of a cDNA encoding murine coagulation factor X. Thrombosis and Haemostasis, 80(1), 87-91.

Cloning and characterization of a cDNA encoding murine coagulation factor X. / Liang, Zhong; Cooper, Adrian; DeFord, Melanie E.; Carmeliet, Peter; Collen, Desire; Castellino, Francis J.; Rosen, Elliot.

In: Thrombosis and Haemostasis, Vol. 80, No. 1, 07.1998, p. 87-91.

Research output: Contribution to journalArticle

Liang, Z, Cooper, A, DeFord, ME, Carmeliet, P, Collen, D, Castellino, FJ & Rosen, E 1998, 'Cloning and characterization of a cDNA encoding murine coagulation factor X', Thrombosis and Haemostasis, vol. 80, no. 1, pp. 87-91.
Liang Z, Cooper A, DeFord ME, Carmeliet P, Collen D, Castellino FJ et al. Cloning and characterization of a cDNA encoding murine coagulation factor X. Thrombosis and Haemostasis. 1998 Jul;80(1):87-91.
Liang, Zhong ; Cooper, Adrian ; DeFord, Melanie E. ; Carmeliet, Peter ; Collen, Desire ; Castellino, Francis J. ; Rosen, Elliot. / Cloning and characterization of a cDNA encoding murine coagulation factor X. In: Thrombosis and Haemostasis. 1998 ; Vol. 80, No. 1. pp. 87-91.
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