Cloning and characterization of a proliferation-associated cytokine-inducible protein, CIP29

Seiji Fukuda, Ding Wen Wu, Kenneth Stark, Louis M. Pelus

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

We identified a novel erythropoietin (Epo)-induced protein (CIP29) in lysates of human UT-7/Epo leukemia cells using two-dimensional gel analysis and cloned its full-length cDNA. CIP29 contains 210 amino acids with a predicted MW of 24 kDa, and has a N-terminal SAP DNA-binding motif. CIP29 expression was higher in cancer and fetal tissues than in normal adult tissues. CIP29 mRNA expression is cytokine regulated in hematopoietic cells, being up-regulated by Epo in UT7/Epo cells, and by thrombopoietin (Tpo), FLT3 ligand (FL) and stem cell factor (SCF) in primary human CD34+ cells. Up-regulation of CIP29 in UT7/Epo cells by Epo was associated with cell cycle progression but not with antiapoptosis. Epo withdrawal reduced CIP29 expression concomitant with cell cycle arrest. Overexpression of CIP29-GFP in HEK293 cells enhances cell cycle progression. CIP29 appears to be a new cytokine regulated protein involved in normal and cancer cell proliferation.

Original languageEnglish (US)
Pages (from-to)593-600
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume292
Issue number3
DOIs
StatePublished - Jan 1 2002

Keywords

  • Apoptosis
  • Cell cycle
  • Cytokine-induced protein
  • SAP protein

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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