Cloning and characterization of Exodus, a novel β-chemokine

Robert Hromas, Patrick W. Gray, David Chantry, Ronald Godiska, Mitchell Krathwohl, Kenneth Fife, Graeme I. Bell, Jun Takeda, Susan Aronica, Michael Gordon, Scott Cooper, Hal Broxmeyer, Michael Klemsz

Research output: Contribution to journalArticle

161 Citations (Scopus)

Abstract

Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 α also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 α, MIP-1 β, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 α) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent β-chemokine.

Original languageEnglish
Pages (from-to)3315-3322
Number of pages8
JournalBlood
Volume89
Issue number9
StatePublished - May 1 1997

Fingerprint

Cloning
Chemokines
Organism Cloning
Bioactivity
Inflammation Mediators
Chemokine CCL5
T-cells
Lymphocytes
Cell proliferation
Chemotaxis
Sequence Homology
Hematopoietic Stem Cells
Viruses
Infiltration
Lipopolysaccharides
Monocytes
Assays
Blood Cells
Leukocytes
Blood

ASJC Scopus subject areas

  • Hematology

Cite this

Hromas, R., Gray, P. W., Chantry, D., Godiska, R., Krathwohl, M., Fife, K., ... Klemsz, M. (1997). Cloning and characterization of Exodus, a novel β-chemokine. Blood, 89(9), 3315-3322.

Cloning and characterization of Exodus, a novel β-chemokine. / Hromas, Robert; Gray, Patrick W.; Chantry, David; Godiska, Ronald; Krathwohl, Mitchell; Fife, Kenneth; Bell, Graeme I.; Takeda, Jun; Aronica, Susan; Gordon, Michael; Cooper, Scott; Broxmeyer, Hal; Klemsz, Michael.

In: Blood, Vol. 89, No. 9, 01.05.1997, p. 3315-3322.

Research output: Contribution to journalArticle

Hromas, R, Gray, PW, Chantry, D, Godiska, R, Krathwohl, M, Fife, K, Bell, GI, Takeda, J, Aronica, S, Gordon, M, Cooper, S, Broxmeyer, H & Klemsz, M 1997, 'Cloning and characterization of Exodus, a novel β-chemokine', Blood, vol. 89, no. 9, pp. 3315-3322.
Hromas R, Gray PW, Chantry D, Godiska R, Krathwohl M, Fife K et al. Cloning and characterization of Exodus, a novel β-chemokine. Blood. 1997 May 1;89(9):3315-3322.
Hromas, Robert ; Gray, Patrick W. ; Chantry, David ; Godiska, Ronald ; Krathwohl, Mitchell ; Fife, Kenneth ; Bell, Graeme I. ; Takeda, Jun ; Aronica, Susan ; Gordon, Michael ; Cooper, Scott ; Broxmeyer, Hal ; Klemsz, Michael. / Cloning and characterization of Exodus, a novel β-chemokine. In: Blood. 1997 ; Vol. 89, No. 9. pp. 3315-3322.
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