Abstract
Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 α also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 α, MIP-1 β, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 α) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent β-chemokine.
| Original language | English |
|---|---|
| Pages (from-to) | 3315-3322 |
| Number of pages | 8 |
| Journal | Blood |
| Volume | 89 |
| Issue number | 9 |
| State | Published - May 1 1997 |
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ASJC Scopus subject areas
- Hematology
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Cloning and characterization of Exodus, a novel β-chemokine. / Hromas, Robert; Gray, Patrick W.; Chantry, David; Godiska, Ronald; Krathwohl, Mitchell; Fife, Kenneth; Bell, Graeme I.; Takeda, Jun; Aronica, Susan; Gordon, Michael; Cooper, Scott; Broxmeyer, Hal; Klemsz, Michael.
In: Blood, Vol. 89, No. 9, 01.05.1997, p. 3315-3322.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Cloning and characterization of Exodus, a novel β-chemokine
AU - Hromas, Robert
AU - Gray, Patrick W.
AU - Chantry, David
AU - Godiska, Ronald
AU - Krathwohl, Mitchell
AU - Fife, Kenneth
AU - Bell, Graeme I.
AU - Takeda, Jun
AU - Aronica, Susan
AU - Gordon, Michael
AU - Cooper, Scott
AU - Broxmeyer, Hal
AU - Klemsz, Michael
PY - 1997/5/1
Y1 - 1997/5/1
N2 - Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 α also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 α, MIP-1 β, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 α) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent β-chemokine.
AB - Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue. Some chemokines such as MIP-1 α also inhibit hematopoietic progenitor cell proliferation. Recently, three chemokines, MIP-1 α, MIP-1 β, and RANTES, have been found to significantly decrease human immunodeficiency virus production from infected T cells. We report here the cloning and characterization of a novel human chemokine termed Exodus for its chemotactic properties. This novel chemokine is distantly related to other chemokines (28% homology with MIP-1 α) and shares several biological activities. Exodus is expressed preferentially in lymphocytes and monocytes, and its expression is markedly upregulated by mediators of inflammation such as tumor necrosis factor or lipopolysaccharide. Purified synthetic Exodus was found to inhibit proliferation of myeloid progenitors in colony formation assays. Exodus also stimulated chemotaxis of peripheral blood mononuclear cells. The sequence homology, expression, and biological activity indicate that Exodus represents a novel divergent β-chemokine.
UR - http://www.scopus.com/inward/record.url?scp=0030905541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0030905541&partnerID=8YFLogxK
M3 - Article
C2 - 9129037
AN - SCOPUS:0030905541
VL - 89
SP - 3315
EP - 3322
JO - Blood
JF - Blood
SN - 0006-4971
IS - 9
ER -