Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis

B. A. Skaggs, J. F. Alexander, C. A. Pierson, K. S. Schweitzer, K. T. Chun, C. Koegel, R. Barbuch, M. Bard

Research output: Contribution to journalArticle

86 Scopus citations

Abstract

The ERGS gene from Saccharomyces cerevisiae was cloned by complementation of an erg5-1 mutation using a negative selection protocol involving screening for nystatin-sensitive transformants. ERG5 is the putative gene encoding the C-22 sterol desaturase required in ergosterol biosynthesis. The functional gene was localized to a 2.15-kb SacI-EcoRI DNA fragment containing an open reading frame of 538 amino acids (aa). ERG5 contains a 10-aa motif consistent with its role as a cytochrome P-450 (CyP450) enzyme and is similar to a number of mammalian CyP450 enzymes. Gene disruption demonstrates that ERG5 is not essential for cell viability.

Original languageEnglish (US)
Pages (from-to)105-109
Number of pages5
JournalGene
Volume169
Issue number1
DOIs
StatePublished - Feb 22 1996

Keywords

  • ERG5
  • Ergosta-5,7-dien-3β-ol
  • Nystatin
  • Recombinant DNA
  • Sterol

ASJC Scopus subject areas

  • Genetics

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