Cloning and expression of the axolotl proto-oncogene ski

David C. Ludolph, Anton W. Neff, Mark A. Parker, Anthony L. Mescher, Rosamund C. Smith, George M. Malacinski

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

In vitro and in vivo overexpression studies have demonstrated that the c-ski proto-oncogene can influence proliferation, morphological transformation and myogenic differentiation. We report the isolation and expression of an axolotl (Ambystoma mexicanum) c-ski (aski) gene. Sequence analysis revealed a high degree of nucleotide and predicted amino acid (AA) homology with mammalian and anuran c-ski, showing the highest conservation to Xenopus laevis c-ski (74% nucleotide and 87% AA). Northern analysis showed that axolotl c-ski is expressed in unfertilized eggs and at increasing levels in embryos from blastula to tadpole stage. c-ski expression was also detected in larval limb muscle and in several stages of regenerating limb blastemas. These data indicate that axolotl c-ski is highly conserved among amphibians and mammals and suggests that it plays a role in urodele embryogenesis and limb regeneration.

Original languageEnglish (US)
Pages (from-to)102-104
Number of pages3
JournalBBA - Gene Structure and Expression
Volume1260
Issue number1
DOIs
StatePublished - Jan 2 1995

Keywords

  • Axolotl
  • c-ski
  • Proto-oncogene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

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    Ludolph, D. C., Neff, A. W., Parker, M. A., Mescher, A. L., Smith, R. C., & Malacinski, G. M. (1995). Cloning and expression of the axolotl proto-oncogene ski. BBA - Gene Structure and Expression, 1260(1), 102-104. https://doi.org/10.1016/0167-4781(94)00194-8