Cloning and expression of the axolotl proto-oncogene ski

David C. Ludolph, Anton W. Neff, Mark A. Parker, Anthony Mescher, Rosamund C. Smith, George M. Malacinski

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

In vitro and in vivo overexpression studies have demonstrated that the c-ski proto-oncogene can influence proliferation, morphological transformation and myogenic differentiation. We report the isolation and expression of an axolotl (Ambystoma mexicanum) c-ski (aski) gene. Sequence analysis revealed a high degree of nucleotide and predicted amino acid (AA) homology with mammalian and anuran c-ski, showing the highest conservation to Xenopus laevis c-ski (74% nucleotide and 87% AA). Northern analysis showed that axolotl c-ski is expressed in unfertilized eggs and at increasing levels in embryos from blastula to tadpole stage. c-ski expression was also detected in larval limb muscle and in several stages of regenerating limb blastemas. These data indicate that axolotl c-ski is highly conserved among amphibians and mammals and suggests that it plays a role in urodele embryogenesis and limb regeneration.

Original languageEnglish
Pages (from-to)102-104
Number of pages3
JournalBiochimica et Biophysica Acta - Gene Structure and Expression
Volume1260
Issue number1
DOIs
StatePublished - Jan 2 1995

Fingerprint

Ambystoma mexicanum
Proto-Oncogenes
Cloning
Organism Cloning
Nucleotides
Amino Acids
Mammals
Extremities
Muscle
Conservation
Genes
Blastula
Xenopus laevis
Amphibians
Embryonic Development
Ovum
Larva
Sequence Analysis
Regeneration
Embryonic Structures

Keywords

  • Axolotl
  • c-ski
  • Proto-oncogene

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics
  • Molecular Biology
  • Structural Biology

Cite this

Cloning and expression of the axolotl proto-oncogene ski. / Ludolph, David C.; Neff, Anton W.; Parker, Mark A.; Mescher, Anthony; Smith, Rosamund C.; Malacinski, George M.

In: Biochimica et Biophysica Acta - Gene Structure and Expression, Vol. 1260, No. 1, 02.01.1995, p. 102-104.

Research output: Contribution to journalArticle

Ludolph, David C. ; Neff, Anton W. ; Parker, Mark A. ; Mescher, Anthony ; Smith, Rosamund C. ; Malacinski, George M. / Cloning and expression of the axolotl proto-oncogene ski. In: Biochimica et Biophysica Acta - Gene Structure and Expression. 1995 ; Vol. 1260, No. 1. pp. 102-104.
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