Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion

Christof Zitt, Andrea Zobel, Alexander Obukhov, Christian Harteneck, Frank Kalkbrenner, Andreas Lückhoff, Günter Schultz

Research output: Contribution to journalArticle

312 Citations (Scopus)

Abstract

Depletion of intracellular calcium stores generates a signal that activates Ca2+-permeable channels in the plasma membrane. We have identified a human cDNA, TRPC1A, from a human fetal brain cDNA library. TRPC1A is homologous to the cation channels trp and trpl in Drosophila and is a splice variant of the recently identified cDNA Htrp-1. Expression of TRPC1A in CHO cells induced nonselective cation currents with similar permeabilities for Na+, Ca2+, and Cs+. The currents were activated by intracellular infusion of myo inositol-1,4,5-trisphosphate or thapsigargin. Expression of TRPC1A significantly enhanced increases in the intracellular free calcium concentration induced by Ca2+ restitution after prolonged depletion. Similar results were obtained in Sf9 cells. We conclude that TRPC1A encodes a Ca2+-permeable cation channel activated by depletion of intracellular calcium stores.

Original languageEnglish (US)
Pages (from-to)1189-1196
Number of pages8
JournalNeuron
Volume16
Issue number6
DOIs
StatePublished - Jun 1996
Externally publishedYes

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Calcium Channels
Cations
Organism Cloning
Calcium
Complementary DNA
Sf9 Cells
Inositol 1,4,5-Trisphosphate
Thapsigargin
CHO Cells
Gene Library
Drosophila
Permeability
Cell Membrane
Brain

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion. / Zitt, Christof; Zobel, Andrea; Obukhov, Alexander; Harteneck, Christian; Kalkbrenner, Frank; Lückhoff, Andreas; Schultz, Günter.

In: Neuron, Vol. 16, No. 6, 06.1996, p. 1189-1196.

Research output: Contribution to journalArticle

Zitt, C, Zobel, A, Obukhov, A, Harteneck, C, Kalkbrenner, F, Lückhoff, A & Schultz, G 1996, 'Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion', Neuron, vol. 16, no. 6, pp. 1189-1196. https://doi.org/10.1016/S0896-6273(00)80145-2
Zitt, Christof ; Zobel, Andrea ; Obukhov, Alexander ; Harteneck, Christian ; Kalkbrenner, Frank ; Lückhoff, Andreas ; Schultz, Günter. / Cloning and functional expression of a human Ca2+-permeable cation channel activated by calcium store depletion. In: Neuron. 1996 ; Vol. 16, No. 6. pp. 1189-1196.
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