Cloning, developmental expression, and evidence for alternative splicing of the murine tuberous sclerosis (TSC2) gene product

Kyung Keun Kim, Laura Pajak, He Wang, Loren Field

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Tuberous sclerosis (TS) is a genetically heterogeneous disease characterized by the widespread appearance of nonmalignant growths that affect multiple organ systems. A TS disease-determining gene, located at 16p133 and designated TSC2, has recently been cloned. In this report, the murine TSC2 homologue was cloned and characterized. cDNA clones encompassing the entire murine TSC2 transcript were isolated. Sequence analysis revealed a high degree of homology between the deduced amino acid sequence of the murine and human gene products. Northern blot surveys demonstrated widespread TSC2 expression which was subject to developmental regulation in a tissue-specific manner. Although high levels of TSC2 transcripts were observed in many adult tissues, protein analyses are required to determine whether functional tuberin protein is synthesized. Reverse transcription-polymerase chain reaction analyses identified at least six regions of alternative splicing, several of which modified putative regulatory motifs in the deduced amino acid structure of the TSC2 protein.

Original languageEnglish
Pages (from-to)515-526
Number of pages12
JournalCellular and Molecular Biology Research
Volume41
Issue number6
StatePublished - 1995

Fingerprint

Tuberous Sclerosis
Cloning
Alternative Splicing
Organism Cloning
Genes
Tissue
Amino Acids
Proteins
Polymerase chain reaction
Transcription
Northern Blotting
Reverse Transcription
Sequence Analysis
Amino Acid Sequence
Complementary DNA
Clone Cells
Polymerase Chain Reaction
Growth

Keywords

  • Antioncogenes
  • Cardiac regeneration
  • Terminal differentiation
  • Tumor suppressors

ASJC Scopus subject areas

  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology

Cite this

Cloning, developmental expression, and evidence for alternative splicing of the murine tuberous sclerosis (TSC2) gene product. / Keun Kim, Kyung; Pajak, Laura; Wang, He; Field, Loren.

In: Cellular and Molecular Biology Research, Vol. 41, No. 6, 1995, p. 515-526.

Research output: Contribution to journalArticle

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