Cloning, expression, and catalytic mechanism of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart

Y. Y P Wo, M. M. Zhou, P. Stevis, J. P. Davis, Zhong-Yin Zhang, R. L. Van Etten

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Abstract

The first representative of a group of mammalian, low molecular weight phosphotyrosyl protein phosphatases was cloned, sequenced and expressed in Escherichia coli. Using a 61-mer oligonucleotide probe based on the amino acid sequence of the purified enzyme, several overlapping cDNA clones were isolated from a bovine heart cDNA library. A full-length clone was obtained consisting of a 27-bp 5' noncoding region, an open reading frame encoding the expected 157 amino acid protein, and an extensive 3' nontranslated sequence. The identification of the clone as full length was consistent with results obtained in mRNA blotting experiments using poly(A)+ mRNA from bovine heart. The coding sequence was placed downstream of a bacteriophage T7 promoter, and protein was expressed in E. coli. The expressed enzyme was soluble, and catalytically active and was readily isolated and purified. The recombinant protein had the expected M(r) of 18 000 (estimated by SDS-PAGE), and it showed cross-reactivity with antisera that had been raised against both the bovine heart and the human placenta enzymes. The amino acid sequence of the N-terminal region of the expressed protein showed that methionine had been removed, resulting in a sequence identical to that of the enzyme isolated from the bovine tissue, with the exception that the N-terminal alanine of the protein from tissue is acetylated. A kinetically competent phosphoenzyme intermediate was trapped from a phosphatase-catalyzed reaction. Using 31P NMR, the covalent intermediate was identified as a cysteinyl phosphate. By analogy with the nomenclature used for serine esterases, these enzymes may be called cysteine phosphatases.

Original languageEnglish (US)
Pages (from-to)1712-1721
Number of pages10
JournalBiochemistry
Volume31
Issue number6
StatePublished - 1992
Externally publishedYes

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Protein Tyrosine Phosphatases
Cloning
Organism Cloning
Molecular Weight
Molecular weight
Enzymes
Clone Cells
Phosphoric Monoester Hydrolases
Amino Acids
Escherichia coli
Amino Acid Sequence
Proteins
Tissue
Bacteriophage T7
Messenger RNA
Bacteriophages
Oligonucleotide Probes
Terminology
Gene Library
Recombinant Proteins

ASJC Scopus subject areas

  • Biochemistry

Cite this

Wo, Y. Y. P., Zhou, M. M., Stevis, P., Davis, J. P., Zhang, Z-Y., & Van Etten, R. L. (1992). Cloning, expression, and catalytic mechanism of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart. Biochemistry, 31(6), 1712-1721.

Cloning, expression, and catalytic mechanism of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart. / Wo, Y. Y P; Zhou, M. M.; Stevis, P.; Davis, J. P.; Zhang, Zhong-Yin; Van Etten, R. L.

In: Biochemistry, Vol. 31, No. 6, 1992, p. 1712-1721.

Research output: Contribution to journalArticle

Wo, Y. Y P ; Zhou, M. M. ; Stevis, P. ; Davis, J. P. ; Zhang, Zhong-Yin ; Van Etten, R. L. / Cloning, expression, and catalytic mechanism of the low molecular weight phosphotyrosyl protein phosphatase from bovine heart. In: Biochemistry. 1992 ; Vol. 31, No. 6. pp. 1712-1721.
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