Cloning of a Variant Epidermal Growth Factor Receptor

T. Moriai, M. S. Kobrin, Murray Korc

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The sequences encoding the full-length epidermal growth factor receptor (EGFR) were cloned from a cDNA library prepared from T3M4 cells. A transition (G to A) was identified at codon 497 of EGFR cDNA, resulting in the substitution of a lysine for an arginine. The same substitution was identified by sequencing cDNA derived from 3 of 7 additional human pancreatic cancer cell lines, one endometrial cancer cell line, and one lung cancer cell line, but not in A43 1 cells. Variant EGFR was always co-expressed with wild-type EGFR. Both sequences were present in genomic DNA from two cell lines expressing the variant receptor and in DNA from normal (3 of 7 individuals) human lymphocytes. These findings indicate that there are two alleles in this region of the EGFR gene, and that expression of variant EGFR is a common occurrence in normal and cancerous cells.

Original languageEnglish (US)
Pages (from-to)1034-1039
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume191
Issue number3
DOIs
StatePublished - Mar 31 1993
Externally publishedYes

Fingerprint

Cloning
Epidermal Growth Factor Receptor
Organism Cloning
Cell Line
Cells
Complementary DNA
erbB-1 Genes
Substitution reactions
Endometrial Neoplasms
Pancreatic Neoplasms
Gene Library
Lymphocytes
Codon
DNA
Lysine
Arginine
Lung Neoplasms
Alleles
Gene Expression
Genes

ASJC Scopus subject areas

  • Molecular Biology
  • Biophysics
  • Biochemistry

Cite this

Cloning of a Variant Epidermal Growth Factor Receptor. / Moriai, T.; Kobrin, M. S.; Korc, Murray.

In: Biochemical and Biophysical Research Communications, Vol. 191, No. 3, 31.03.1993, p. 1034-1039.

Research output: Contribution to journalArticle

Moriai, T. ; Kobrin, M. S. ; Korc, Murray. / Cloning of a Variant Epidermal Growth Factor Receptor. In: Biochemical and Biophysical Research Communications. 1993 ; Vol. 191, No. 3. pp. 1034-1039.
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