Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1

Ananda L. Roy, Hong Du, Polly D. Gregor, Carl D. Novina, Ernest Martinez, Robert G. Roeder

Research output: Contribution to journalArticle

163 Citations (Scopus)

Abstract

The transcription factor TFII-I has been shown to bind independently to two distinct promoter elements, a pyrimidine-rich initiator (Inr) and a recognition site (E-box) for upstream stimulatory factor 1 (USF1), and to stimulate USF1 binding to both of these sites. Here we describe the isolation of a cDNA encoding TFII-I and demonstrate that the corresponding 120 kDa polypeptide, when expressed ectopically, is capable of binding to both Inr and E-box elements. The primary structure of TFII-I reveals novel features that include six directly repeated 90 residue motifs that each possess a potential helix-loop/span-helix homology. These unique structural features suggest that TFII-I may have the capacity for multiple protein-protein and, potentially, multiple protein-DNA interactions. Consistent with this hypothesis and with previous in vitro studies, we further demonstrate that ectopic TFII-I and USF1 can act synergistically, and in some cases independently, to activate transcription in vivo through both Inr and the E-box elements of the adenovirus major late promoter. We also describe domains of USF1 that are necessary for its independent and synergistic activation functions.

Original languageEnglish (US)
Pages (from-to)7091-7104
Number of pages14
JournalEMBO Journal
Volume16
Issue number23
StatePublished - Dec 1 1997

Fingerprint

Upstream Stimulatory Factors
Cloning
E-Box Elements
Organism Cloning
Carrier Proteins
TFII Transcription Factors
Proteins
Transcription
Adenoviridae
Complementary DNA
Chemical activation
Peptides
DNA

Keywords

  • Adenovirus major late promoter
  • E-box
  • Inr
  • Transcription factor TFII-I
  • Upstream activation factor 1

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

Cite this

Roy, A. L., Du, H., Gregor, P. D., Novina, C. D., Martinez, E., & Roeder, R. G. (1997). Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1. EMBO Journal, 16(23), 7091-7104.

Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1. / Roy, Ananda L.; Du, Hong; Gregor, Polly D.; Novina, Carl D.; Martinez, Ernest; Roeder, Robert G.

In: EMBO Journal, Vol. 16, No. 23, 01.12.1997, p. 7091-7104.

Research output: Contribution to journalArticle

Roy, AL, Du, H, Gregor, PD, Novina, CD, Martinez, E & Roeder, RG 1997, 'Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1', EMBO Journal, vol. 16, no. 23, pp. 7091-7104.
Roy AL, Du H, Gregor PD, Novina CD, Martinez E, Roeder RG. Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1. EMBO Journal. 1997 Dec 1;16(23):7091-7104.
Roy, Ananda L. ; Du, Hong ; Gregor, Polly D. ; Novina, Carl D. ; Martinez, Ernest ; Roeder, Robert G. / Cloning of an inr- and E-box-binding protein, TFII-I, that interacts physically and functionally with USF1. In: EMBO Journal. 1997 ; Vol. 16, No. 23. pp. 7091-7104.
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