Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells

Robert Hromas, Hal Broxmeyer, Chang Kim, Harikrishna Nakshatri, Kent Christopherson, Mohd Azam, Yong Hao Hou

Research output: Contribution to journalArticle

135 Citations (Scopus)

Abstract

Chemokines are a family of related proteins that regulate leukocyte infiltration into inflamed tissue and play important roles in many disease processes. Chemokines are divided into two major groups, CC or CXC, based on their sequence around the amino terminal cysteines. We report the PCR cloning of a novel human chemokine termed BRAK for its initial isolation from breast and kidney cells. This novel chemokine is distantly related to other CXC chemokines (30% identity with MIP-2α and β) and shares several biological activities. BRAK is expressed ubiquitously and highly in normal tissue. However, it was expressed in only 2 of 18 cancer cell lines. BRAK is located on human chromosome 5q31.

Original languageEnglish
Pages (from-to)703-706
Number of pages4
JournalBiochemical and Biophysical Research Communications
Volume255
Issue number3
DOIs
StatePublished - Feb 24 1999

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CXC Chemokines
Cloning
Chemokines
Organism Cloning
Tissue
Human Chromosomes
Chromosomes
Bioactivity
Infiltration
Cysteine
Breast
Leukocytes
Cells
Kidney
Cell Line
Polymerase Chain Reaction
Neoplasms
Proteins

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Cloning of BRAK, a novel divergent CXC chemokine preferentially expressed in normal versus malignant cells. / Hromas, Robert; Broxmeyer, Hal; Kim, Chang; Nakshatri, Harikrishna; Christopherson, Kent; Azam, Mohd; Hou, Yong Hao.

In: Biochemical and Biophysical Research Communications, Vol. 255, No. 3, 24.02.1999, p. 703-706.

Research output: Contribution to journalArticle

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